Production of L-DOPA and dopamine in recombinant bacteria bearing the Vitreoscilla hemoglobin gene

Given the well‐established beneficial effects of Vitreoscilla hemoglobin (VHb) on heterologous organisms, the potential of this protein for the production of L‐DOPA and dopamine in two bacteria, Citrobacter freundii and Erwinia herbicola, was investigated. The constructed recombinants bearing the VH...

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Bibliographic Details
Published in:Biotechnology journal 2009-07, Vol.4 (7), p.1077-1088
Main Authors: Kurt, Asli Giray, Aytan, Emel, Ozer, Ufuk, Ates, Burhan, Geckil, Hikmet
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Citrobacter freundii
Citrobacter freundii - genetics
Citrobacter freundii - metabolism
Dopamine
Dopamine - biosynthesis
Dopamine - genetics
Erwinia - genetics
Erwinia - metabolism
Erwinia herbicola
Hemeproteins - genetics
Hemeproteins - metabolism
L-DOPA
Levodopa - biosynthesis
Levodopa - genetics
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Truncated Hemoglobins - genetics
Truncated Hemoglobins - metabolism
Tyrosine - metabolism
Tyrosine phenol-lyase
Tyrosine Phenol-Lyase - metabolism
Vitreoscilla
Vitreoscilla hemoglobin
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Summary:Given the well‐established beneficial effects of Vitreoscilla hemoglobin (VHb) on heterologous organisms, the potential of this protein for the production of L‐DOPA and dopamine in two bacteria, Citrobacter freundii and Erwinia herbicola, was investigated. The constructed recombinants bearing the VHb gene (vgb+) had substantially higher levels of cytoplasmic L‐DOPA (112 mg/L for C. freundii and 97 mg/L for E. herbicola) than their respective hosts (30.4 and 33.8 mg/L) and the vgb– control strains (35.6 and 35.8 mg/L). Further, the vgb+ recombinants of C. freundii and E. herbicola had 20‐fold and about two orders of magnitude higher dopamine levels than their hosts, repectively. The activity of tyrosine phenol‐lyase, the enzyme converting L‐tyrosine to L‐DOPA, was well‐correlated to cytoplasmic L‐DOPA levels. As cultures aged, higher tyrosine phenol‐lyase activity of the vgb+ strains was more apparent.
ISSN:1860-6768
1860-7314
DOI:10.1002/biot.200900130