KLK5 and KLK7, two members of the human tissue kallikrein family, are differentially expressed in lung cancer

Emerging data indicate that serine proteases of the kallikrein family ( KLK) are implicated in various human diseases, including carcinoma; however, kallikrein gene expression has never been investigated in lung cancer. Using RT-PCR and Western blotting, we demonstrated the expression of both KLK5 a...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-04, Vol.329 (4), p.1260-1266
Main Authors: Planque, Chris, de Monte, Michèle, Guyetant, Serge, Rollin, Jérôme, Desmazes, Claire, Panel, Valentine, Lemarié, Etienne, Courty, Yves
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Female
Gene Expression Regulation, Neoplastic
Human kallikrein
Humans
Kallikreins
Lung cancer
Lung Neoplasms - genetics
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Male
Middle Aged
Polymerase Chain Reaction
Real-time PCR
RNA, Messenger - genetics
RNA, Messenger - metabolism
Serine Endopeptidases - genetics
Serine Endopeptidases - metabolism
Serine protease
Tumor marker
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Summary:Emerging data indicate that serine proteases of the kallikrein family ( KLK) are implicated in various human diseases, including carcinoma; however, kallikrein gene expression has never been investigated in lung cancer. Using RT-PCR and Western blotting, we demonstrated the expression of both KLK5 and KLK7, and their respective proteins (hK5 and hK7) in tumoral and nontumoral lung tissues. Quantitative gene expression was then analyzed in a cohort of 56 patients with non-small cell lung cancer by real-time RT-PCR. KLK5 expression is significantly more expressed in squamous cell carcinoma than in matched nonmalignant lung tissue ( P = 0.02), whereas expression of KLK7 was decreased in adenocarcinoma ( P = 0.003). Multivariate analysis revealed diverse correlations between the KLK5 and KLK7 expression levels in nonmalignant and malignant tissues, and clinical parameters, including histotype, metastatic status, and grade. Our findings provide new insight into kallikrein gene expression in hormone-independent carcinoma. Altogether, our results suggest that variability in KLK5 and KLK7 gene expression might be involved in lung tumorigenesis and useful for clinical purposes.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.02.100