Application of a new chemiluminescence method for the determination of glucose-6-phosphate dehydrogenase activity in healthy and enzyme-deficient individuals

• Published in: Journal of pharmacological and toxicological methods 2005-03, Vol.51 (2), p.169-174
• Main Authors: Gumuslu, Saadet, Yucel, Gultekin, Sarikcioglu, Sureyya Bilmen, Serteser, Mustafa
• Format: Article
• Language: English
• Subjects: Area under curve; Chemiluminescence; Erythrocytes - enzymology; Female; G-6-PD deficiency; Glucosephosphate Dehydrogenase - blood; Glucosephosphate Dehydrogenase - genetics; Glucosephosphate Dehydrogenase Deficiency - blood; Glucosephosphate Dehydrogenase Deficiency - genetics; Heterozygote; Homozygote; Humans; Luminescent Measurements - methods; Male; Reproducibility of Results; Sensitivity and Specificity
• ISSN: 1056-8719; 1873-488X
• DOI: 10.1016/j.vascn.2004.09.002

Introduction:A chemiluminescence (CL) technique, which determines the glucose-6-phosphate dehydrogenase (G-6-PD) activities in healthy, heterozygous, and completely enzyme-deficient individuals was applied. Methods: CL intensities were detected for 4 h at 15-min intervals in each sample with or without addition of G-6-PD substrates into the reaction mixture. Results:The results revealed an inverse correlation to the reference UV method (Zinkham method; r=−0.80). Furthermore, the CL assay was able to detect G-6-PD activities as low as 0.2 IU/gHb, which was not possible by the UV method. Discussion:In conclusion, we believe that this method offers a new diagnostic tool for the detection of G-6-PD activities in enzyme-deficient individuals and, because of its increased sensitivity, makes it amenable for determining the effects of different pharmaceutical agents on G-6-PD activity in tissue or cell cultures.