High frequency of somatic missense mutation of BRCA2 in female breast cancer from Taiwan

• Published in: Cancer letters 2005-04, Vol.220 (2), p.177-184
• Main Authors: Chen, Fang-Ming, Hou, Ming-Feng, Chang, Mei-Yin, Wang, Jaw-Yuan, Hsieh, Jan-Sing, Ou-yang, Fu, Huang, Tsung-Jen, Lin, Shiu-Ru
• Format: Article
• Language: English
• Subjects: Adult; Aged; BRCA2; Breast cancer; Breast Neoplasms - epidemiology; Breast Neoplasms - genetics; Case-Control Studies; Cell cycle; Deoxyribonucleic acid; DNA; DNA Mutational Analysis; Exons; Female; Genes; Genes, BRCA2; Humans; Incidence; Middle Aged; Mortality; Mutation; Mutation, Missense; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Somatic mutation; Studies; Taiwan; Taiwan - epidemiology; Tumorigenesis; Tumors
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2004.10.024

Somatic mutation of BRCA2 has been thought to be rare in breast cancers, though common allelic deletions in the BRCA2 locus (13q12-q13) imply an important role of somatic mutation in these tumors. Reasons of the reported rare incidence could be related to very few studies focusing on the mutational analysis of BRCA2 in sporadic tumors. The mutational status of the BRCA2 gene in exon11, the largest exon harboring the RAD51 interacting BRC domains which are critical for BRCA2 function, was screened by polymerase chain reaction/single strand conformation polymorphism (PCR-SSCP) analysis followed by direct sequencing. Tumor and paired normal tissue sample from 175 patients unselected for family history or age were taken after mastectomy for breast cancer and evaluated. There were 20 mutations of BRCA2 gene in exon 11 in 15 cases (15/175, 8.6%). Most mutations we identified were point mutation (19/20, 95%), except for one nucleotide insertion. Furthermore, among these mutations, missense mutations comprised 80% (16/20) of the BRCA2 mutations. All mutations we found were novel mutation after searched in the BIC database. There were three recurrent mutations at codon 1904; and two recurrent mutations at 1907, 1936, 1937 and 1968, respectively. The mutations were associated with ductal carcinoma in situ ( P=0.038) and borderline with low histological grade ( P=0.072). Besides, there were three cases possessing multiple mutations in the region we studied and one of them demonstrated aggressive lymph node metastasis. These findings implicated that somatic mutations of BRCA2 genes may play a significant role in the pathogenesis of breast carcinoma in Taiwan. In addition, those events were associated with some early clinicopathological features.