Performance Evaluation of a High-field Orbitrap Mass Analyzer

A new design of the Orbitrap™ mass analyzer is presented. Higher frequencies of ion oscillations and hence higher resolving power over fixed acquisition time are achieved by decreasing the gap between the inner and outer Orbitrap electrodes, thus providing higher field strength for a given voltage....

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2009-08, Vol.20 (8), p.1391-1396
Main Authors: Makarov, Alexander, Denisov, Eduard, Lange, Oliver
Format: Article
Language:English
Subjects:
Analytical Chemistry
Bioinformatics
Biotechnology
Chemistry
Chemistry and Materials Science
Exact sciences and technology
Field strength
Focus: The Orbitrap
Instruments, apparatus, components and techniques common to several branches of physics and astronomy
Mass spectrometers and related techniques
Mass spectrometry
Organic Chemistry
Performance evaluation
Physics
Proteins
Proteomics
Resolution
Space charge
Spectrometric and optical methods
Tolerances
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Summary:A new design of the Orbitrap™ mass analyzer is presented. Higher frequencies of ion oscillations and hence higher resolving power over fixed acquisition time are achieved by decreasing the gap between the inner and outer Orbitrap electrodes, thus providing higher field strength for a given voltage. Experimental results confirm maximum FWHM resolving power in excess of 350,000 at m/z 524 and 600,000 at m/z 195, isotopic resolution of proteins above 40 kDa, and a single-shot dynamic range of 25,000. It was also found that mass shifts in the new design depend very little on space charge inside the analyzer. This performance was achieved using higher voltages and by careful balancing of construction tolerances and operation parameters, which appeared to vary in narrower ranges of tuning than for a standard Orbitrap analyzer. This work is devoted to experimental characterization of a novel, high-field Orbitrap mass analyzer with a decreased gap between electrodes.
ISSN:1044-0305
1879-1123
DOI:10.1016/j.jasms.2009.01.005