S100B secretion in acute brain slices: modulation by extracellular levels of Ca(2+) and K (+)

Hippocampal slices have been widely used to investigate electrophysiological and metabolic neuronal parameters, as well as parameters of astroglial activity including protein phosphorylation and glutamate uptake. S100B is an astroglial-derived protein, which extracellularly plays a neurotrophic acti...

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Published in:Neurochemical research 2009-09, Vol.34 (9), p.1603-1611
Main Authors: Nardin, Patrícia, Tortorelli, Lucas, Quincozes-Santos, André, de Almeida, Lúcia Maria V, Leite, Marina C, Thomazi, Ana Paula, Gottfried, Carmem, Wofchuk, Susana T, Donato, Rosario, Gonçalves, Carlos-Alberto
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
Calcium - pharmacology
Calcium Channel Blockers - pharmacology
Extracellular Space - metabolism
Glutamic Acid - metabolism
Glutathione - metabolism
Hippocampus - drug effects
Hippocampus - secretion
Male
Nerve Growth Factors - secretion
Phosphopyruvate Hydratase - metabolism
Potassium - metabolism
Potassium - pharmacology
Rats
Rats, Wistar
S100 Calcium Binding Protein beta Subunit
S100 Proteins - secretion
Verapamil - pharmacology
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Summary:Hippocampal slices have been widely used to investigate electrophysiological and metabolic neuronal parameters, as well as parameters of astroglial activity including protein phosphorylation and glutamate uptake. S100B is an astroglial-derived protein, which extracellularly plays a neurotrophic activity during development and excitotoxic insult. Herein, we characterized S100B secretion in acute hippocampal slices exposed to different concentrations of K(+) and Ca(2+) in the extracellular medium. Absence of Ca(2+) and/or low K(+) (0.2 mM KCl) caused an increase in S100B secretion, possibly by mobilization of internal stores of Ca(2+). In contrast, high K(+) (30 mM KCl) or calcium channel blockers caused a decrease in S100B secretion. This study suggests that exposure of acute hippocampal slices to low- and high-K(+) could be used as an assay to evaluate astrocyte activity by S100B secretion: positively regulated by low K(+) (possibly involving mobilization of internal stores of Ca(2+)) and negatively regulated by high-K(+) (likely secondary to influx of K(+)).
ISSN:1573-6903
DOI:10.1007/s11064-009-9949-0