Mouse ES cell lines show a variable degree of chondrogenic differentiation in vitro

Pluripotent mouse embryonic stem (ES) cells differentiate in vitro spontaneously into cell types of all three primary germ layers when cultivated as cell aggregates, so-called ‘embryoid bodies’. Many reports have shown that this system recapitulates cellular developmental processes and gene expressi...

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Bibliographic Details
Published in:Cell biology international 2005-02, Vol.29 (2), p.139-146
Main Authors: Kramer, Jan, Hegert, Claudia, Hargus, Gunnar, Rohwedel, Jürgen
Format: Article
Language:English
Subjects:
Alcian Blue - chemistry
Animals
Cartilage - cytology
Cartilage - metabolism
Cell Differentiation
Cell Line
Chondrocytes - cytology
Chondrocytes - metabolism
Chondrogenesis
Coloring Agents
Differentiation potential
Embryo, Mammalian - cytology
Gene Expression
In vitro differentiation
Mesoderm - cytology
Mice - embryology
Mouse embryonic stem cells
Pluripotency
Pluripotent Stem Cells - cytology
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Summary:Pluripotent mouse embryonic stem (ES) cells differentiate in vitro spontaneously into cell types of all three primary germ layers when cultivated as cell aggregates, so-called ‘embryoid bodies’. Many reports have shown that this system recapitulates cellular developmental processes and gene expression patterns of early embryogenesis. During ES cell differentiation, efficient and directed differentiation into a specific cell type is influenced by many parameters, for example, the batch of the serum used or the application of growth factors and signalling molecules. Because all ES cell lines are considered to be pluripotent, one should not expect remarkable differences regarding their spontaneous differentiation efficiencies. However, here we show that different ES cell lines exhibit a variable degree of spontaneous chondrogenic differentiation indicating that lines with a specific differentiation capacity could be selected. This is an important aspect if ES cells are applied for tissue regeneration.
ISSN:1065-6995
1095-8355
DOI:10.1016/j.cellbi.2004.10.003