Levels of L-Selectin (CD62L) on Human Leukocytes in Disseminated Cryptococcosis With and Without Associated HIV-1 Infection

Patients with disseminated cryptococcosis typically have measurable levels of cryptococcal polysaccharide in serum samples but minimal leukocyte infiltration into infected tissues. In vitro data have shown that cryptococcal polysaccharide induces L-selectin (CD62L) shedding from leukocytes. To asses...

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Bibliographic Details
Published in:The Journal of infectious diseases 2005-04, Vol.191 (8), p.1361-1367
Main Authors: Jackson, Lydgia A., Drevets, Douglas A., Dong, Zhao-Ming, Greenfield, Ronald A., Murphy, Juneann W.
Format: Article
Language:English
Subjects:
Adult
Antigens
Biological and medical sciences
Cohort Studies
Cryptococcosis
Cryptococcosis - blood
Cryptococcosis - complications
Cryptococcosis - metabolism
Fundamental and applied biological sciences. Psychology
Fungi
HIV
HIV 1
HIV Infections - blood
HIV Infections - complications
HIV Infections - virology
Human immunodeficiency virus 1
Human viral diseases
Humans
Infections
Infectious diseases
L-Selectin - analysis
L-Selectin - blood
Leukocytes
Leukocytes - metabolism
Medical sciences
Microbiology
Middle Aged
Miscellaneous
Monocytes
Monocytes - metabolism
Neutrophils
Neutrophils - metabolism
Polysaccharides
Solubility
T lymphocytes
T-Lymphocytes - metabolism
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Virology
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Description
Summary:Patients with disseminated cryptococcosis typically have measurable levels of cryptococcal polysaccharide in serum samples but minimal leukocyte infiltration into infected tissues. In vitro data have shown that cryptococcal polysaccharide induces L-selectin (CD62L) shedding from leukocytes. To assess shedding in vivo, we compared leukocyte L-selectin levels in human immunodeficiency virus (HIV) type 1–negative and -positive subjects with and without circulating cryptococcal polysaccharide. Results showed that subjects with cryptococcal polysaccharide in serum samples have significantly lower percentages of neutrophils, monocytes, and CD3+ T cells with L-selectin on their surfaces than do healthy subjects, regardless of HIV status. There was significantly more soluble L-selectin in serum samples from subjects with cryptococcosis than in those from uninfected subjects. Reduced L-selectin levels on leukocytes in subjects with circulating cryptococcal polysaccharide and increased serum levels of soluble L-selectin indicates that surface L-selectin shedding is a mechanism that likely explains reduced leukocyte extravasation into infected tissues of patients with disseminated cryptococcosis
ISSN:0022-1899
1537-6613
DOI:10.1086/428949