Characterization of Escherichia coli O86 O-antigen gene cluster and identification of O86-specific genes

Escherichia coli O86 belongs to the enteropathogenic E. coli (EPEC) group, some strains of which are pathogens of humans, wild birds and farm animals. The O-antigen gene cluster of E. coli O86 was amplified by long-range PCR using primers based on the housekeeping genes galF and gnd, and then sequen...

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Bibliographic Details
Published in:Veterinary microbiology 2005-04, Vol.106 (3), p.241-248
Main Authors: Feng, Lu, Han, Weiqing, Wang, Quan, Bastin, David A., Wang, Lei
Format: Article
Language:English
Subjects:
Animals
bacterial antigens
Base Sequence
bird diseases
Carbohydrate Sequence
disease detection
DNA, Bacterial - chemistry
E. coli O127
E. coli O86
enteropathogenic Escherichia coli
EPEC
Escherichia coli
Escherichia coli - genetics
Escherichia infections
farmed animal species
Genes, Bacterial
human diseases
Humans
microbial genetics
Molecular Sequence Data
Molecular typing
Multigene Family
nucleotide sequences
O Antigens - chemistry
O Antigens - genetics
PCR assay
phylogeny
polymerase chain reaction
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
rapid methods
Sequence Homology
Serotyping
Shigella
Shigella - genetics
strains
wild birds
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Summary:Escherichia coli O86 belongs to the enteropathogenic E. coli (EPEC) group, some strains of which are pathogens of humans, wild birds and farm animals. The O-antigen gene cluster of E. coli O86 was amplified by long-range PCR using primers based on the housekeeping genes galF and gnd, and then sequenced. Genes involved in GDP-Fuc and N-acetyl-galactosamine (GalNAc) synthesis and genes encoding glycosyltransferases, O-unit flippase and O-antigen polymerase were identified on the basis of homology. By screening against 186 E. coli and Shigella-type strains, two genes specific to E. coli O86 were identified. A polymerase chain reaction (PCR) assay, based on the specific O-antigen genes identified here, could be used for the rapid detection of E. coli O86 in environmental and clinical samples. The relationship between E. coli O86 and O127 was also determined by comparing the two O-antigen gene clusters.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2004.12.021