A comprehensive radiation hybrid map of the bovine genome comprising 5593 loci

A bovine whole genome 7000-rad radiation hybrid (RH) panel, SUNbRH 7000-rad, was constructed to build a high-resolution RH map. The Shirakawa–USDA linkage map served as a scaffold to construct a framework map of 3216 microsatellites on which 2377 ESTs were ordered. The resulting RH map provided esse...

Full description

Saved in:
Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2005-04, Vol.85 (4), p.413-424
Main Authors: Itoh, Tomohito, Watanabe, Toshio, Ihara, Naoya, Mariani, Paola, Beattie, Craig W., Sugimoto, Yoshikazu, Takasuga, Akiko
Format: Article
Language:English
Subjects:
Animals
bacterial artificial chromosomes
Biological and medical sciences
Cattle
Cattle - genetics
chromosome mapping
Classical genetics, quantitative genetics, hybrids
Comparative map
EST
Expressed Sequence Tags
Fundamental and applied biological sciences. Psychology
Genes. Genome
genetic markers
Genetics of eukaryotes. Biological and molecular evolution
Genome
Humans
loci
Methods, theories and miscellaneous
Microsatellite
microsatellite repeats
Microsatellite Repeats - genetics
Molecular and cellular biology
Molecular genetics
physical chromosome mapping
Radiation hybrid map
Radiation Hybrid Mapping
radiation hybrid maps
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A bovine whole genome 7000-rad radiation hybrid (RH) panel, SUNbRH 7000-rad, was constructed to build a high-resolution RH map. The Shirakawa–USDA linkage map served as a scaffold to construct a framework map of 3216 microsatellites on which 2377 ESTs were ordered. The resulting RH map provided essentially complete coverage across the genome, with 1 cR 7000 corresponding to 114 kb, and a cattle–human comparative map of 1716 bovine genes and sequences annotated in the human genome, which covered 79 and 72% of the bovine and human genomes, respectively. We then integrated the bovine RH and comparative maps with BAC fingerprint information in http://www.bcgsc.ca/lab/mapping/bovine to construct a detailed, BAC-based physical map covering a reported 40-cM quantitative trait locus region for intramuscular fat or “marbling” on BTA 4. In summary, the new, high-resolution SUNbRH 7000-rad, comparative, Shirakawa–USDA linkage, and BAC fingerprint maps provide a set of genomic tools for fine mapping regions of interest in cattle.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2004.12.007