Light-dependent expression of superoxide dismutase from cyanobacterium Synechocystis sp. strain PCC 6803

The oxygenic phototrophic cyanobacterium Synechocystis sp. strain PCC 6803 inevitably evolves superoxide during photosynthesis. Synechocystis 6803 contains only one type of superoxide dismutase, designated as SodB; therefore, this protein plays an important role in preventing oxidative damages cause...

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Bibliographic Details
Published in:Archives of microbiology 2005-03, Vol.183 (3), p.218-223
Main Authors: KIM, Jae-Hyun, KYONG HOON SUH
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - analysis
Bacteriology
Biological and medical sciences
Blotting, Northern
Cyanophyta
Electrophoresis, Gel, Two-Dimensional
Enzymes
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Genes
Light
Microbiology
Miscellaneous
Photosynthesis
RNA, Bacterial - analysis
RNA, Messenger
Superoxide Dismutase - genetics
Superoxide Dismutase - metabolism
Synechocystis
Synechocystis - enzymology
Synechocystis - genetics
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Summary:The oxygenic phototrophic cyanobacterium Synechocystis sp. strain PCC 6803 inevitably evolves superoxide during photosynthesis. Synechocystis 6803 contains only one type of superoxide dismutase, designated as SodB; therefore, this protein plays an important role in preventing oxidative damages caused by light. Because there was no direct evidence that SodB in Synechocystis 6803 could be regulated by light, the relationship between SodB and light was investigated in the present study. The activity of SodB from the cells grown in continuous light culture was about 3.5-fold higher than that from the cells cultivated in continuous dark. Illumination maximally activated SodB within 12 h. The level of sodB mRNA increased 12-fold by light, and that of SodB protein proportionally. Therefore, the expression and activity of SodB from Synechocystis 6803 were dependent on the light.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-005-0766-9