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Differential Gene Expression Profiling of Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells by DNA Microarray

Mesenchymal stem cells (MSCs) retain both self‐renewal and multilineage differentiation capabilities. Despite wide therapeutic potential, many aspects of human MSCs, particularly the molecular parameters to define the stemness, remain largely unknown. Using high‐density oligonucleotide micro‐arrays,...

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Bibliographic Details
Published in:Stem cells (Dayton, Ohio) Ohio), 2005-04, Vol.23 (4), p.584-593
Main Authors: Jeong, Ju Ah, Hong, Seung Hyun, Gang, Eun Ji, Ahn, Chiyoung, Hwang, Soo Han, Yang, Il Ho, Han, Hoon, Kim, Hoeon
Format: Article
Language:English
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Summary:Mesenchymal stem cells (MSCs) retain both self‐renewal and multilineage differentiation capabilities. Despite wide therapeutic potential, many aspects of human MSCs, particularly the molecular parameters to define the stemness, remain largely unknown. Using high‐density oligonucleotide micro‐arrays, we obtained the differential gene expression profile between a fraction of mononuclear cells of human umbilical cord blood (UCB) and its MSC subpopulation. Of particular interest was a subset of 47 genes preferentially expressed at 50‐fold or higher in MSCs, which could be regarded as a molecular foundation of human MSCs. This subset contains numerous genes encoding collagens, other extracellular matrix or related proteins, cytokines or growth factors, and cytoskeleton‐associated proteins but very few genes for membrane and nuclear proteins. In addition, a direct comparison of this microarray‐generated transcriptome with the published serial analysis of gene expression data suggests that a molecular context of UCB‐derived MSCs is more or less similar to that of bone marrow–derived cells. Altogether, our results will provide a basis for studies on molecular mechanisms controlling core properties of human MSCs.
ISSN:1066-5099
1549-4918
DOI:10.1634/stemcells.2004-0304