Large-scale general collection of wild-plant DNA in Mustang, Nepal

The deposit of DNA samples of wild plants that correspond to voucher specimens is highly informative and greatly enhances the value of the herbarium specimens. The Society of Himalayan Botany (SHB), Tokyo, has assembled general collections of flowering plants of the Sino-Himalayan region for more th...

Full description

Saved in:
Bibliographic Details
Published in:Journal of plant research 2005-02, Vol.118 (1), p.57-60
Main Authors: Tsukaya, H. (National Inst. for Basic Biology, Okazaki, Aichi (Japan)), Iokawa, Y, Kondo, M, Ohba, H
Format: Article
Language:English
Subjects:
Botany
Botany - methods
Deoxyribonucleic acid
DNA
DNA, Plant - isolation & purification
Flora
Flowering plants
GENETIC RESOURCES
Genetic testing
HIMALAYAN REDION
Magnoliopsida - genetics
Microorganisms
Nepal
Preservation, Biological - methods
SPERMATOPHYTA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The deposit of DNA samples of wild plants that correspond to voucher specimens is highly informative and greatly enhances the value of the herbarium specimens. The Society of Himalayan Botany (SHB), Tokyo, has assembled general collections of flowering plants of the Sino-Himalayan region for more than 40 years. In a trial of the collection of these types of bioresources for use in basic research, we adopted FTA cards, which have recently been used for large-scale collection of DNA of humans, micro-organisms and viruses, for the general collection of DNA samples of wild plants during a botanical expedition in Mustang, Nepal, in 2003. Three hundred and fifty-rive plant specimens from Mustang, Nepal, were collected along with the corresponding DNA samples. Examination of the quality of the DNA samples by PCR demonstrated the utility of the collection system. The identification of all of the specimens collected, as well as data from the specimens, will be presented on the Flora of Nepal Database website (http://ti.um.u-tokyo.ac.jp/default.htm), which is open to the public. The DNA resources will be identified on the website and distributed openly by the SHB to researchers worldwide for basic research.
ISSN:0918-9440
1618-0860
DOI:10.1007/s10265-005-0196-4