Hydrogen peroxide sensor based on horseradish peroxidase immobilized on a silver nanoparticles/cysteamine/gold electrode

A third-generation hydrogen peroxide biosensor was prepared by immobilizing horseradish peroxidase (HRP) on a gold electrode modified with silver nanoparticles. A freshly-cleaned gold electrode was first immersed in a cysteamine-ethanol solution, and then silver nanoparticles were immobilized on the...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2005-03, Vol.381 (6), p.1179-1185
Main Authors: Ren, Chunbo, Song, Yonghai, Li, Zhuang, Zhu, Guoyi
Format: Article
Language:English
Subjects:
Biosensing Techniques - methods
Cysteamine - chemistry
Electrochemistry - methods
Electrodes
Gold - chemistry
Horseradish Peroxidase - metabolism
Hydrogen Peroxide - analysis
Microscopy, Atomic Force
Nanotechnology
Particle Size
Reproducibility of Results
Sensitivity and Specificity
Silver - chemistry
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Summary:A third-generation hydrogen peroxide biosensor was prepared by immobilizing horseradish peroxidase (HRP) on a gold electrode modified with silver nanoparticles. A freshly-cleaned gold electrode was first immersed in a cysteamine-ethanol solution, and then silver nanoparticles were immobilized on the cysteamine monolayer, and finally HRP was adsorbed onto the surfaces of the silver nanoparticles. This self-assemble process was examined via atomic force microscopy (AFM). The immobilized horseradish peroxidase exhibited an excellent electrocatalytic response toward the reduction of hydrogen peroxide. The linear range of the biosensor was 3.3 microM to 9.4 mM, and the detection limit was estimated to be 0.78 microM. Moreover, the biosensor exhibited a fast response, high sensitivity, good reproducibility, and long-term stability.
ISSN:1618-2642
DOI:10.1007/s00216-004-3032-0