Induction of Both Membrane-Bound and Soluble HLA-G Expression in Active Human Cytomegalovirus Infection

BackgroundAlteration of HLA expression or cytokine production plays a crucial role in the pathogenesis of human cytomegalovirus (HCMV) infection. HLA-G has been suggested to be involved in HCMV infection, and modulation of HLA-G expression by interferon (IFN)–γ and interleukin (IL)–10 has been repor...

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Bibliographic Details
Published in:The Journal of infectious diseases 2009-09, Vol.200 (5), p.820-826
Main Authors: Yan, Wei-Hua, Lin, Aifen, Chen, Bao-Guo, Chen, Shi-Yong
Format: Article
Language:English
Subjects:
Biological and medical sciences
Case-Control Studies
Cell Membrane - chemistry
Child, Preschool
Cytokines
Cytomegalovirus
Cytomegalovirus infections
Cytomegalovirus Infections - immunology
Enzyme-Linked Immunosorbent Assay - methods
Female
Flow Cytometry - methods
Fundamental and applied biological sciences. Psychology
Histocompatibility Antigens Class I - analysis
Histocompatibility Antigens Class I - blood
HLA Antigens - analysis
HLA Antigens - blood
HLA-G Antigens
Human cytomegalovirus
Humans
Infant
Infections
Infectious diseases
Interferon-gamma - blood
Interleukin-10 - blood
Male
Medical sciences
Microbiology
Miscellaneous
Molecules
Monocytes
Monocytes - chemistry
Monocytes - immunology
Natural killer cells
Neurons
T lymphocytes
Virology
Viruses
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Summary:BackgroundAlteration of HLA expression or cytokine production plays a crucial role in the pathogenesis of human cytomegalovirus (HCMV) infection. HLA-G has been suggested to be involved in HCMV infection, and modulation of HLA-G expression by interferon (IFN)–γ and interleukin (IL)–10 has been reported. However, the clinical relevance of HLA-G in HCMV infection remains unknown MethodsThe study included 75 patients with active HCMV infection (age range, 1–4.5 years) and 150 sex- and age-matched healthy control subjects (age range, 1–5 years). HLA-G expression in peripheral monocytes from patients (n=38) and control subjects (n=20) was analyzed using flow cytometry. Plasma levels of soluble HLA-G (in 75 patients and 150 control subjects), IL-10 (in 75 patients and 40 control subjects), and IFN-γ (in 75 patients and 40 control subjects) were determined using enzyme-linked immunosorbent assay ResultsThe mean percentage of HLA-G–positive monocytes among patients with active HCMV infection was dramatically increased, compared with that among healthy control subjects (6.33% vs 1.64%; P
ISSN:0022-1899
1537-6613
DOI:10.1086/604733