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Induction of Both Membrane-Bound and Soluble HLA-G Expression in Active Human Cytomegalovirus Infection
BackgroundAlteration of HLA expression or cytokine production plays a crucial role in the pathogenesis of human cytomegalovirus (HCMV) infection. HLA-G has been suggested to be involved in HCMV infection, and modulation of HLA-G expression by interferon (IFN)–γ and interleukin (IL)–10 has been repor...
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| Published in: | The Journal of infectious diseases 2009-09, Vol.200 (5), p.820-826 |
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| creator | Yan, Wei-Hua Lin, Aifen Chen, Bao-Guo Chen, Shi-Yong |
| description | BackgroundAlteration of HLA expression or cytokine production plays a crucial role in the pathogenesis of human cytomegalovirus (HCMV) infection. HLA-G has been suggested to be involved in HCMV infection, and modulation of HLA-G expression by interferon (IFN)–γ and interleukin (IL)–10 has been reported. However, the clinical relevance of HLA-G in HCMV infection remains unknown MethodsThe study included 75 patients with active HCMV infection (age range, 1–4.5 years) and 150 sex- and age-matched healthy control subjects (age range, 1–5 years). HLA-G expression in peripheral monocytes from patients (n=38) and control subjects (n=20) was analyzed using flow cytometry. Plasma levels of soluble HLA-G (in 75 patients and 150 control subjects), IL-10 (in 75 patients and 40 control subjects), and IFN-γ (in 75 patients and 40 control subjects) were determined using enzyme-linked immunosorbent assay ResultsThe mean percentage of HLA-G–positive monocytes among patients with active HCMV infection was dramatically increased, compared with that among healthy control subjects (6.33% vs 1.64%; P |
| doi_str_mv | 10.1086/604733 |
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HLA-G has been suggested to be involved in HCMV infection, and modulation of HLA-G expression by interferon (IFN)–γ and interleukin (IL)–10 has been reported. However, the clinical relevance of HLA-G in HCMV infection remains unknown MethodsThe study included 75 patients with active HCMV infection (age range, 1–4.5 years) and 150 sex- and age-matched healthy control subjects (age range, 1–5 years). HLA-G expression in peripheral monocytes from patients (n=38) and control subjects (n=20) was analyzed using flow cytometry. Plasma levels of soluble HLA-G (in 75 patients and 150 control subjects), IL-10 (in 75 patients and 40 control subjects), and IFN-γ (in 75 patients and 40 control subjects) were determined using enzyme-linked immunosorbent assay ResultsThe mean percentage of HLA-G–positive monocytes among patients with active HCMV infection was dramatically increased, compared with that among healthy control subjects (6.33% vs 1.64%; P<.001). Similarly, significant increases were observed in soluble HLA-G level (median, 54.91 vs 21.32 U/mL; P<.001) and IL-10 level (median, 9.24 vs 1.82 ng/mL; P<.001). Although the expression of IFN-γ was higher in patients with active HCMV infection than in healthy control subjects, the difference was not statistically significant (median, 1254.46 vs 887.05 ng/mL; P=.070). Furthermore, no correlation was established between HLA-G expression and levels of IL-10 or IFN-γ ConclusionsHLA-G expression in monocytes and plasma soluble HLA-G and IL-10 levels were increased during active HCMV infection</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/604733</identifier><identifier>PMID: 19619059</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Oxford: The University of Chicago Press</publisher><subject>Biological and medical sciences ; Case-Control Studies ; Cell Membrane - chemistry ; Child, Preschool ; Cytokines ; Cytomegalovirus ; Cytomegalovirus infections ; Cytomegalovirus Infections - immunology ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; Flow Cytometry - methods ; Fundamental and applied biological sciences. Psychology ; Histocompatibility Antigens Class I - analysis ; Histocompatibility Antigens Class I - blood ; HLA Antigens - analysis ; HLA Antigens - blood ; HLA-G Antigens ; Human cytomegalovirus ; Humans ; Infant ; Infections ; Infectious diseases ; Interferon-gamma - blood ; Interleukin-10 - blood ; Male ; Medical sciences ; Microbiology ; Miscellaneous ; Molecules ; Monocytes ; Monocytes - chemistry ; Monocytes - immunology ; Natural killer cells ; Neurons ; T lymphocytes ; Virology ; Viruses</subject><ispartof>The Journal of infectious diseases, 2009-09, Vol.200 (5), p.820-826</ispartof><rights>Copyright 2009 Infectious Diseases Society of America</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-68cee12a3b2215aa7f5fdeea1e751bd464db185d471df0a5ff0465cd3b43b5693</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21831218$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19619059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, Wei-Hua</creatorcontrib><creatorcontrib>Lin, Aifen</creatorcontrib><creatorcontrib>Chen, Bao-Guo</creatorcontrib><creatorcontrib>Chen, Shi-Yong</creatorcontrib><title>Induction of Both Membrane-Bound and Soluble HLA-G Expression in Active Human Cytomegalovirus Infection</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><description>BackgroundAlteration of HLA expression or cytokine production plays a crucial role in the pathogenesis of human cytomegalovirus (HCMV) infection. HLA-G has been suggested to be involved in HCMV infection, and modulation of HLA-G expression by interferon (IFN)–γ and interleukin (IL)–10 has been reported. However, the clinical relevance of HLA-G in HCMV infection remains unknown MethodsThe study included 75 patients with active HCMV infection (age range, 1–4.5 years) and 150 sex- and age-matched healthy control subjects (age range, 1–5 years). HLA-G expression in peripheral monocytes from patients (n=38) and control subjects (n=20) was analyzed using flow cytometry. Plasma levels of soluble HLA-G (in 75 patients and 150 control subjects), IL-10 (in 75 patients and 40 control subjects), and IFN-γ (in 75 patients and 40 control subjects) were determined using enzyme-linked immunosorbent assay ResultsThe mean percentage of HLA-G–positive monocytes among patients with active HCMV infection was dramatically increased, compared with that among healthy control subjects (6.33% vs 1.64%; P<.001). Similarly, significant increases were observed in soluble HLA-G level (median, 54.91 vs 21.32 U/mL; P<.001) and IL-10 level (median, 9.24 vs 1.82 ng/mL; P<.001). Although the expression of IFN-γ was higher in patients with active HCMV infection than in healthy control subjects, the difference was not statistically significant (median, 1254.46 vs 887.05 ng/mL; P=.070). Furthermore, no correlation was established between HLA-G expression and levels of IL-10 or IFN-γ ConclusionsHLA-G expression in monocytes and plasma soluble HLA-G and IL-10 levels were increased during active HCMV infection</description><subject>Biological and medical sciences</subject><subject>Case-Control Studies</subject><subject>Cell Membrane - chemistry</subject><subject>Child, Preschool</subject><subject>Cytokines</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus infections</subject><subject>Cytomegalovirus Infections - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>Flow Cytometry - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Histocompatibility Antigens Class I - analysis</subject><subject>Histocompatibility Antigens Class I - blood</subject><subject>HLA Antigens - analysis</subject><subject>HLA Antigens - blood</subject><subject>HLA-G Antigens</subject><subject>Human cytomegalovirus</subject><subject>Humans</subject><subject>Infant</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Interferon-gamma - blood</subject><subject>Interleukin-10 - blood</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecules</subject><subject>Monocytes</subject><subject>Monocytes - chemistry</subject><subject>Monocytes - immunology</subject><subject>Natural killer cells</subject><subject>Neurons</subject><subject>T lymphocytes</subject><subject>Virology</subject><subject>Viruses</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkEFv0zAYhi3ExLoC_wDkC9zCPtuxnRy7sK3dikBiSIiL5cT2yEjiYifT9u_n0qo7cvDnw_N8r_S9CL0l8IlAIU4F5JKxF2hGOJOZEIS9RDMASjNSlOUxOonxDgByJuQrdExKQUrg5QzdrgYzNWPrB-wdPvPjb_zF9nXQg83O_DQYrNP77rup7ixerhfZJT5_2AQb43anHfAibd8nNPV6wNXj6Ht7qzt_34Yp4tXg7L_01-jI6S7aN_t_jn5cnN9Uy2z99XJVLdZZkzMYM1E01hKqWU0p4VpLx52xVhMrOalNLnJTk4KbXBLjQHPnIBe8MazOWc1Fyebo4y53E_zfycZR9W1sbNelg_wUlZCcF8Dof0VKKJRUFs9iE3yMwTq1CW2vw6MioLbdq133SXy_T5zq3ppnbV92Ej7sBR0b3blUctPGg0dJwch2zNG7nXcXRx8OPAfKOUhIPNvxNo724cB1-JPOY5Kr5c9f6tv11UV1U31WFXsCYACjcg</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Yan, Wei-Hua</creator><creator>Lin, Aifen</creator><creator>Chen, Bao-Guo</creator><creator>Chen, Shi-Yong</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20090901</creationdate><title>Induction of Both Membrane-Bound and Soluble HLA-G Expression in Active Human Cytomegalovirus Infection</title><author>Yan, Wei-Hua ; Lin, Aifen ; Chen, Bao-Guo ; Chen, Shi-Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-68cee12a3b2215aa7f5fdeea1e751bd464db185d471df0a5ff0465cd3b43b5693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biological and medical sciences</topic><topic>Case-Control Studies</topic><topic>Cell Membrane - chemistry</topic><topic>Child, Preschool</topic><topic>Cytokines</topic><topic>Cytomegalovirus</topic><topic>Cytomegalovirus infections</topic><topic>Cytomegalovirus Infections - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>Flow Cytometry - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histocompatibility Antigens Class I - analysis</topic><topic>Histocompatibility Antigens Class I - blood</topic><topic>HLA Antigens - analysis</topic><topic>HLA Antigens - blood</topic><topic>HLA-G Antigens</topic><topic>Human cytomegalovirus</topic><topic>Humans</topic><topic>Infant</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Interferon-gamma - blood</topic><topic>Interleukin-10 - blood</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecules</topic><topic>Monocytes</topic><topic>Monocytes - chemistry</topic><topic>Monocytes - immunology</topic><topic>Natural killer cells</topic><topic>Neurons</topic><topic>T lymphocytes</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yan, Wei-Hua</creatorcontrib><creatorcontrib>Lin, Aifen</creatorcontrib><creatorcontrib>Chen, Bao-Guo</creatorcontrib><creatorcontrib>Chen, Shi-Yong</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yan, Wei-Hua</au><au>Lin, Aifen</au><au>Chen, Bao-Guo</au><au>Chen, Shi-Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of Both Membrane-Bound and Soluble HLA-G Expression in Active Human Cytomegalovirus Infection</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>200</volume><issue>5</issue><spage>820</spage><epage>826</epage><pages>820-826</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>BackgroundAlteration of HLA expression or cytokine production plays a crucial role in the pathogenesis of human cytomegalovirus (HCMV) infection. HLA-G has been suggested to be involved in HCMV infection, and modulation of HLA-G expression by interferon (IFN)–γ and interleukin (IL)–10 has been reported. However, the clinical relevance of HLA-G in HCMV infection remains unknown MethodsThe study included 75 patients with active HCMV infection (age range, 1–4.5 years) and 150 sex- and age-matched healthy control subjects (age range, 1–5 years). HLA-G expression in peripheral monocytes from patients (n=38) and control subjects (n=20) was analyzed using flow cytometry. Plasma levels of soluble HLA-G (in 75 patients and 150 control subjects), IL-10 (in 75 patients and 40 control subjects), and IFN-γ (in 75 patients and 40 control subjects) were determined using enzyme-linked immunosorbent assay ResultsThe mean percentage of HLA-G–positive monocytes among patients with active HCMV infection was dramatically increased, compared with that among healthy control subjects (6.33% vs 1.64%; P<.001). Similarly, significant increases were observed in soluble HLA-G level (median, 54.91 vs 21.32 U/mL; P<.001) and IL-10 level (median, 9.24 vs 1.82 ng/mL; P<.001). Although the expression of IFN-γ was higher in patients with active HCMV infection than in healthy control subjects, the difference was not statistically significant (median, 1254.46 vs 887.05 ng/mL; P=.070). Furthermore, no correlation was established between HLA-G expression and levels of IL-10 or IFN-γ ConclusionsHLA-G expression in monocytes and plasma soluble HLA-G and IL-10 levels were increased during active HCMV infection</abstract><cop>Oxford</cop><pub>The University of Chicago Press</pub><pmid>19619059</pmid><doi>10.1086/604733</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Case-Control Studies Cell Membrane - chemistry Child, Preschool Cytokines Cytomegalovirus Cytomegalovirus infections Cytomegalovirus Infections - immunology Enzyme-Linked Immunosorbent Assay - methods Female Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Histocompatibility Antigens Class I - analysis Histocompatibility Antigens Class I - blood HLA Antigens - analysis HLA Antigens - blood HLA-G Antigens Human cytomegalovirus Humans Infant Infections Infectious diseases Interferon-gamma - blood Interleukin-10 - blood Male Medical sciences Microbiology Miscellaneous Molecules Monocytes Monocytes - chemistry Monocytes - immunology Natural killer cells Neurons T lymphocytes Virology Viruses |
| title | Induction of Both Membrane-Bound and Soluble HLA-G Expression in Active Human Cytomegalovirus Infection |
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