Multilocus sequence typing reveals that Bacillus cereus strains isolated from clinical infections have distinct phylogenetic origins

Eight strains of Bacillus cereus isolated from bacteremia and soft tissue infections were assigned to seven sequence types (STs) by multilocus sequence typing (MLST). Two strains from different locations had identical STs. The concatenated sequences of the seven STs were aligned with 65 concatenated...

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Bibliographic Details
Published in:FEMS microbiology letters 2005-04, Vol.245 (1), p.179-184
Main Authors: Barker, Margaret, Thakker, Bishan, Priest, Fergus G.
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Alleles
Bacillaceae Infections - microbiology
Bacillus - classification
Bacillus - genetics
Bacillus anthracis
Bacillus cereus
Bacillus cereus - classification
Bacillus cereus - genetics
Bacillus cereus - isolation & purification
Bacillus thuringiensis
Bacteremia
Bacteremia - microbiology
Bacterial Proteins - genetics
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Clone
DNA, Bacterial - analysis
Female
Fundamental and applied biological sciences. Psychology
Genetics
Humans
Male
Microbiology
Middle Aged
MLST
Multilocus sequence typing
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Phylogeny
Sequence Analysis, DNA
Soft Tissue Infections - microbiology
Soft tissues
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Description
Summary:Eight strains of Bacillus cereus isolated from bacteremia and soft tissue infections were assigned to seven sequence types (STs) by multilocus sequence typing (MLST). Two strains from different locations had identical STs. The concatenated sequences of the seven STs were aligned with 65 concatenated sequences from reference STs and a neighbor-joining tree was constructed. Two strains were distantly related to all reference STs. Three strains were recovered in a clade that included Bacillus anthracis, B. cereus and rare Bacillus thuringiensis strains while the other three strains were assigned to two STs that were more closely affiliated to most of the B. thuringiensis STs. We conclude that invasive B. cereus strains do not form a single clone or clonal complex of highly virulent strains.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2005.03.003