Enhanced human osteoblast cell adhesion and proliferation on 316 LS stainless steel by means of CO2 laser surface treatment

An effective and novel technique for improving the biocompatibility of a biograde 316 LS stainless steel through the application of CO2 laser treatment to modify the surface properties of the material is described herein. Different surface properties, such as surface roughness, surface oxygen conten...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2005-04, Vol.73B (1), p.148-156
Main Authors: Hao, L., Lawrence, J., Phua, Y. F., Chian, K. S., Lim, G. C., Zheng, H. Y.
Format: Article
Language:English
Subjects:
316 LS stainless steel
Biocompatible Materials - chemistry
Carbon Dioxide - chemistry
Cell Adhesion
Cell Culture Techniques
Cell Proliferation
CO2 laser
Humans
Hydrogen-Ion Concentration
Ions
Lasers
Microscopy, Electron, Scanning
Models, Theoretical
osteoblast cell adhesion and proliferation
Osteoblasts - cytology
Osteoblasts - metabolism
Oxygen - chemistry
Spectrometry, X-Ray Emission
Stainless Steel - chemistry
Surface Properties
surface roughness
Wettability
wettability characteristics
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Summary:An effective and novel technique for improving the biocompatibility of a biograde 316 LS stainless steel through the application of CO2 laser treatment to modify the surface properties of the material is described herein. Different surface properties, such as surface roughness, surface oxygen content, and surface energy for CO2 laser‐treated 316 LS stainless steel, untreated, and mechanically roughened samples were analyzed, and their effects on the wettability characteristics of the material were studied. It was found that modification of the wettability characteristics of the 316 LS stainless steel following CO2 laser treatment was achieved. This improvement was identified as being mainly due to the change in the polar component of the surface energy. One‐day cell adhesion tests showed that cells not only adhered and spread better, but also grew faster on the CO2 laser‐treated sample than on either the untreated or mechanically roughened sample. Further, compared with the untreated sample, MTT cell proliferation analysis revealed that the mechanically roughed surface resulted in a slight enhancement, and CO2 laser treatment brought about a significant increase in cell proliferation. An increase in the wettability of the 316 LS stainless steel was observed to positively correlate with the cell proliferation. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 73B: 148–156, 2005
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.30194