Heterologous protein production using the Pichia pastoris expression system

The Pichia pastoris expression system is being used successfully for the production of various recombinant heterologous proteins. Recent developments with respect to the Pichia expression system have had an impact on not only the expression levels that can be achieved, but also the bioactivity of va...

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Bibliographic Details
Published in:Yeast (Chichester, England) England), 2005-03, Vol.22 (4), p.249-270
Main Authors: Macauley‐Patrick, Sue, Fazenda, Mariana L., McNeil, Brian, Harvey, Linda M.
Format: Article
Language:English
Subjects:
Bioreactors - microbiology
foreign protein production
Gene Dosage
Gene Expression Regulation, Fungal - physiology
Genetic Markers - physiology
heterologous expression
Industrial Microbiology - methods
methylotrophic
Pichia - genetics
Pichia - metabolism
Pichia pastoris
post‐translational modification
process optimization
Protein Biosynthesis - physiology
Protein Processing, Post-Translational - physiology
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
yeast
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Summary:The Pichia pastoris expression system is being used successfully for the production of various recombinant heterologous proteins. Recent developments with respect to the Pichia expression system have had an impact on not only the expression levels that can be achieved, but also the bioactivity of various heterologous proteins. We review here some of these recent developments, as well as strategies for reducing proteolytic degradation of the expressed recombinant protein at cultivation, cellular and protein levels. The problems associated with post‐translational modifications performed on recombinant proteins by P. pastoris are discussed, including the effects on bioactivity and function of these proteins, and some engineering strategies for minimizing unwanted glycosylations. We pay particular attention to the importance of optimizing the physicochemical environment for efficient and maximal recombinant protein production in bioreactors and the role of process control in optimizing protein production is reviewed. Finally, future aspects of the use of the P. pastoris expression system are discussed with regard to the production of complex membrane proteins, such as G protein‐coupled receptors, and the industrial and clinical importance of these proteins. Copyright © 2005 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
DOI:10.1002/yea.1208