A colorimetric method for detection and quantification of chlorinating activity of hemeperoxidases

A method of detecting and assaying the halogenating activity of hemeperoxidases using the colored substrate, thionin, is reported here. In the presence of suitable amounts of peroxide and chloride, chloroperoxidase chlorinates thionin and bleaches the intense color of this substrate. The kinetics wa...

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Bibliographic Details
Published in:Analytical biochemistry 2006, Vol.348 (1), p.84-86
Main Authors: Manoj, Kelath Murali, Hager, Lowell P.
Format: Article
Language:English
Subjects:
Chlorination assay
Chloroperoxidase
Hemeperoxidase
Peroxidases - analysis
Phenothiazines - chemistry
Sensitivity and Specificity
Spectrophotometry, Ultraviolet - methods
Thionin
Time Factors
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Summary:A method of detecting and assaying the halogenating activity of hemeperoxidases using the colored substrate, thionin, is reported here. In the presence of suitable amounts of peroxide and chloride, chloroperoxidase chlorinates thionin and bleaches the intense color of this substrate. The kinetics was quite similar to that of the established monochlorodimedone assay. Therefore, the thionin assay can be taken as an index of chlorinating activity. This reaction affords an escape from background problems and allows easy processing of large volumes of samples.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2005.10.014