Physical and genetic mapping of amplified fragment length polymorphisms and the leaf rust resistance Lr3 gene on chromosome 6BL of wheat

• Published in: Theoretical and applied genetics 2006, Vol.112 (2), p.251-257
• Main Authors: Dieguez, M.J, Altieri, E, Ingala, L.R, Perera, E, Sacco, F, Naranjo, T
• Format: Article
• Language: English
• Subjects: amplified fragment length polymorphism; Biological and medical sciences; C-banding; Chromosome Banding; Chromosome Deletion; Chromosome Mapping; Chromosomes, Plant - genetics; Classical genetics, quantitative genetics, hybrids; deletion lines; disease resistance; Fundamental and applied biological sciences. Psychology; genes; Genes, Plant - genetics; Genetic Linkage; Genetic Markers; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genomics; leaves; lines; Lr3 gene; Methods, theories and miscellaneous; Physical Chromosome Mapping; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Leaves - genetics; Plant Leaves - microbiology; plant pathogenic fungi; Polymerase Chain Reaction; Polymorphism, Genetic - genetics; Pteridophyta, spermatophyta; Puccinia recondita; rust diseases; Triticum - genetics; Triticum - microbiology; Triticum aestivum; Vegetals; wheat
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-005-0122-0

The Argentinian wheat cultivar Sinvalocho MA carries the Lr3 gene for leaf rust resistance on distal chromosome 6BL. In this cultivar, 33 spontaneous susceptible lines were isolated and cytogenetically characterized by C-banding. The analysis revealed deletions on chromosome 6BL in most lines. One line was nulli-6B, two lines were ditelo 6BS, two, three, and ten lines had long terminal deletions of 40, 30, and 20%, respectively, three lines showed very small terminal deletions, and one line had an intercalary deletion of 11%. Physical mapping of 55 amplified fragment length polymorphism (AFLP) markers detected differences between deletions and led to the division of 6BL into seven bins delimited by deletion breakpoints. The most distal bin, with a length smaller than 5% of 6BL, contained 22 AFLP markers and the Lr3 gene. Polymorphism for nine AFLPs between Sinvalocho MA and the rust leaf susceptible cultivar Gamma 6 was used to construct a linkage map of Lr3. This gene is at a genetic distance of 0.9 cM from a group of seven closely linked AFLPs. The location of the gene in a high recombinogenic region indicated a physical distance of approximately 1 Mb to the markers.