Up to 21-day banked red blood cells collected by apheresis and stored for 14 days after automated wash at different times of storage

• Published in: Vox sanguinis 2006-01, Vol.90 (1), p.40-44
• Main Authors: Grabmer, C., Holmberg, J., Popovsky, M., Amann, E., Schönitzer, D., Falaize, S., Hanske, H., Pages, E., Nussbaumer, W.
• Format: Article
• Language: English
• Subjects: allergic reactions; Blood Component Removal - instrumentation; Blood Component Removal - methods; Blood Preservation - methods; Erythrocyte Transfusion; Erythrocytes - cytology; Humans; IgA deficiency; neonatal; potassium; Time Factors; washed RBC
• ISSN: 0042-9007; 1423-0410
• DOI: 10.1111/j.1423-0410.2005.00719.x

Background and Objectives  A closed‐system technology (ACP‐215, Haemonetics, Braintree, MA) enables automated washing and extended storage of frozen red blood cells (RBC). This technology was applied to wash banked RBC for removal of undesirable protein and metabolites before transfusion. We studied protein and metabolite depletion as well as RBC metabolism and viability up to 14 days postwash with regard to various pre‐storage times. Materials and Methods  Thirty RBC units were collected by means of apheresis and subdivided into three arms based on prewash storage time period (6 days/group 1, 14 days/group 2, 21 days/group 3). Wash efficacy (protein depletion, IgA), RBC metabolism (pH, lactate, potassium, haemolysis) and cell viability (ATP) were analysed immediately and 14 days after washing. Results  Total protein and IgA postwash were lowered by automated wash in all groups and uniformly met EC guidelines. Potassium (mmol/l) was below 1·2 mmol/l postwash and significantly below prewash values in all groups, even after 14 days of storage (prewash vs. postwash; P