Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia

A cDNA clone encoding a putative Bop1 homologous protein was identified in Giardia lamblia. Since Bop1 is a nucleolar protein involved in rRNA processing, thereby controlling the cell cycle, we investigated components of cell cycle control in G. lamblia by identifying the protein(s) that interact wi...

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Bibliographic Details
Published in:Parasitology research (1987) 2006-01, Vol.98 (2), p.138-144
Main Authors: Kim, J, Goo, S. Y, Chung, H. J, Yang, H. -W, Yong, T. -S, Lee, K. -H, Park, S. -J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
antigens
Biological and medical sciences
cDNA libraries
cell cycle
clones
Cloning, Molecular
complementary DNA
Cytoskeletal Proteins - chemistry
Cytoskeletal Proteins - genetics
Cytoskeletal Proteins - metabolism
cytoskeleton
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Gene Library
General aspects
General aspects and techniques. Study of several systematic groups. Models
genes
Giardia lamblia
Giardia lamblia - genetics
Giardia lamblia - growth & development
Giardia lamblia - metabolism
giardin protein
Immunoprecipitation
Invertebrates
Molecular Sequence Data
polyclonal antibodies
Protozoan Proteins - chemistry
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
rats
ribosomal RNA
Two-Hybrid System Techniques
yeasts
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Summary:A cDNA clone encoding a putative Bop1 homologous protein was identified in Giardia lamblia. Since Bop1 is a nucleolar protein involved in rRNA processing, thereby controlling the cell cycle, we investigated components of cell cycle control in G. lamblia by identifying the protein(s) that interact with Bop 1. Through an immunoaffinity column made with polyclonal antibodies specific to the recombinant Bop1 of G. lamblia, a pool of proteins was obtained from the crude extracts of Giardia and then used as antigens to immunize rats. By employing the resultant sera for cDNA library immunoscreening, we isolated cDNA clones encoding an immunopurified protein, which turned out to contain the gene for β-giardin, a Giardia-specific cytoskeletal protein. The interaction between Bop1 and β-giardin was confirmed via two different methods, yeast two-hybrid assay and coimmunoprecipitation.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-005-0040-8