Comparison of in vitro replication features of H7N3 influenza viruses from wild ducks and turkeys: potential implications for interspecies transmission

In previous work, it was shown that turkey H7N3 influenza viruses, presumably derived 'in toto' from interspecies transmission of duck viruses in Northern Italy, had only 2 aa differences in haemagglutinin and a few amino acid differences as well as a 23 aa deletion in neuraminidase compar...

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Bibliographic Details
Published in:Journal of general virology 2006, Vol.87 (1), p.171-175
Main Authors: Giannecchini, S, Campitelli, L, Calzoletti, L, De Marco, M.A, Azzi, A, Donatelli, I
Format: Article
Language:English
Subjects:
Anatidae
Animals
Animals, Wild - virology
avian influenza
binding capacity
Biological and medical sciences
Bird Diseases - transmission
Bird Diseases - virology
cell fusion
Cell Line
cell lines
disease transmission
dogs
Ducks
erythrocytes
Fundamental and applied biological sciences. Psychology
Hemagglutinin Glycoproteins, Influenza Virus - genetics
in vitro studies
Influenza A virus
Influenza A virus - genetics
Influenza A virus - pathogenicity
Influenza in Birds - epidemiology
Influenza in Birds - transmission
Influenza in Birds - virology
Influenza virus
kidneys
Microbiology
Miscellaneous
Poultry Diseases - transmission
Poultry Diseases - virology
receptors
strains
Turkeys
Virology
Virus Replication
wild birds
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Summary:In previous work, it was shown that turkey H7N3 influenza viruses, presumably derived 'in toto' from interspecies transmission of duck viruses in Northern Italy, had only 2 aa differences in haemagglutinin and a few amino acid differences as well as a 23 aa deletion in neuraminidase compared with duck viruses. Here, the replication of these duck and turkey viruses in Madin-Darby canine kidney cells was investigated with respect to virus-cell fusion and viral elution from red blood cells. Duck viruses showed similar receptor-binding properties to turkey viruses but possessed a higher pH of fusion activation than the turkey viruses. Conversely, turkey viruses were not able to elute from red blood cells. These data confirm that neuraminidase-stalk deletion impairs the release of virions from cells and also confirm existence of naturally occurring viruses with different pH fusion activities, raising the possibility that these features may play a role in the evolution of influenza viruses in different hosts.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.81187-0