use of a recombinant baculovirus expressing a chitinase from the hard tick Haemaphysalis longicornis and its potential application as a bioacaricide for tick control

Baculoviruses are specific insect pathogens used as selective biological insecticides on lepidopteran insects. We have tested a recombinant baculovirus expressing a chitinase gene for its efficacy as a tick bioacaricide. The recombinant Autographa californica multiple nuclear polyhedrosis virus expr...

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Bibliographic Details
Published in:Parasitology research (1987) 2006, Vol.98 (2), p.111-118
Main Authors: Assenga, Severine P, You, Myungjo, Shy, Chee Huey, Yamagishi, Junya, Sakaguchi, Takeshi, Zhou, Jinlin, Kibe, Michael K, Xuan, Xuenan, Fujisaki, Kozo
Format: Article
Language:English
Subjects:
Animals
Baculoviridae
Baculoviridae - enzymology
Baculoviridae - genetics
Biological and medical sciences
Cells, Cultured
chitinase
Chitinases - genetics
Chitinases - metabolism
flumethrin
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Invertebrates
Ixodidae - drug effects
Ixodidae - growth & development
Ixodidae - virology
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
Pyrethrins - pharmacology
Recombinant Proteins - metabolism
Spodoptera - virology
temperature
Tick Control - methods
ticks
UV-radiation
Vectors. Intermediate hosts
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Summary:Baculoviruses are specific insect pathogens used as selective biological insecticides on lepidopteran insects. We have tested a recombinant baculovirus expressing a chitinase gene for its efficacy as a tick bioacaricide. The recombinant Autographa californica multiple nuclear polyhedrosis virus expressing a chitinase enzyme (AcMNPV-CHT1) from the hard tick, Haemaphysalis longicornis, was constructed and found to have a novel bioacaricidal effect against ticks. The recombinant baculovirus was used to express the chitinase enzyme in Spodoptera frugiperda (Sf9) insect cells. Topical application of the supernatant harvested from the insect cell culture was found to cause mortality in nymphal ticks of H. longicornis. High temperature (>30°C) and infrared radiation affected the chitinase enzyme activity and recombinant baculovirus infectivity by reducing the speed of tick killing by 60%. A mixture of recombinant virus and chitinase was found to kill ticks faster (p
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-005-0007-9