Comparison and phylogenetic analysis of cowpox viruses isolated from cats and humans in Fennoscandia

Cowpox virus (CPXV), a member of the genus Orthopoxvirus (OPV), has reservoirs in small mammals and may cause disease in humans, felidae and other animals. In this study we compared CPXVs isolated from humans and cats in Fennoscandia by restriction enzyme and DNA sequence analysis. The HindIII restr...

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Bibliographic Details
Published in:Archives of virology 2009-08, Vol.154 (8), p.1293-1302
Main Authors: Hansen, Hilde, Okeke, Malachy Ifeanyi, Nilssen, Øivind, Traavik, Terje
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cats - virology
CHO Cells - virology
Comparative analysis
Cowpox
Cowpox - veterinary
Cowpox - virology
Cowpox virus
Cowpox virus - classification
Cowpox virus - genetics
Cowpox virus - isolation & purification
Cricetinae
Cricetulus
Cytokines
Denmark
Felidae
Finland
Fundamental and applied biological sciences. Psychology
Genes, Viral
Genetic Variation
Hemagglutinins
Hemagglutinins, Viral - genetics
Host range
Humans
Infectious Diseases
Medical Microbiology
Microbiology
Miscellaneous
Molecular Sequence Data
Norway
Nucleotide sequence
Original Article
Orthopoxvirus
Phylogenetics
Phylogeny
Receptors, Tumor Necrosis Factor - genetics
Sequence analysis
Sweden
Viral Proteins - genetics
Virology
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Summary:Cowpox virus (CPXV), a member of the genus Orthopoxvirus (OPV), has reservoirs in small mammals and may cause disease in humans, felidae and other animals. In this study we compared CPXVs isolated from humans and cats in Fennoscandia by restriction enzyme and DNA sequence analysis. The HindIII restriction profiles clearly distinguished geographically distinct CPXV isolates, whereas only minor differences were found between the profiles of geographically linked isolates. The complete gene sequences encoding the cytokine response modifier B, the hemagglutinin and the Chinese hamster ovary host range protein were determined for the same isolates and included in phylogenetic analysis. By including representative OPV sequences from GenBank, detailed comparative analyses were performed showing pronounced heterogeneity among CPXVs compared to members of other OPV species. However, a close relationship between the Norwegian (3 of 4 isolates) and Swedish isolates was detected, whereas the isolate from Finland was more closely related to a Russian isolate for all three genes compared. We infer that the investigated CPXVs have distinct evolutionary histories in different rodent lineages.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-009-0442-5