Consensus sequence L/PKSSLL mimics crucial epitope on Loop III of Taiwan cobra cardiotoxin

Phage display is effective in screening peptides that mimic venom’s neutralizing epitopes. A phage display cyclized heptapeptide library (C7C library) was panned with purified divalent antivenin IgG, which neutralizes Naja naja atra venom (NAV) and Bungarus multicinctus venom (BMV). The selected hep...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2009-09, Vol.387 (3), p.617-622
Main Authors: Wang, Ping-Chieh, Loh, Kah-Sin, Lin, Shih-Ting, Chien, Tzu-Ling, Chiang, Jen-Ron, Hsieh, Wen-Chin, Miao, Bor-Lin, Su, Cheng-Fu, Yang, Wen-Jen
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies - immunology
Bungarus multicinctus
Cardiotoxin
Cobra Cardiotoxin Proteins - chemistry
Cobra Cardiotoxin Proteins - genetics
Cobra Cardiotoxin Proteins - immunology
Consensus Sequence
Epitope
Epitope Mapping
Epitopes - chemistry
Epitopes - genetics
Epitopes - immunology
Female
Mice
Mice, Inbred BALB C
Molecular Mimicry
Molecular Sequence Data
Naja naja atra
Neutralization Tests
Peptide Library
Phage display
Protein Structure, Secondary
Taiwan cobra ( Naja naja atra)
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Summary:Phage display is effective in screening peptides that mimic venom’s neutralizing epitopes. A phage display cyclized heptapeptide library (C7C library) was panned with purified divalent antivenin IgG, which neutralizes Naja naja atra venom (NAV) and Bungarus multicinctus venom (BMV). The selected heptapeptide sequences were aligned with known protein sequences of NAV and BMV in GenBank. One of the four consensus sequences, L/PKSSLL, mimicked the crucial epitope on Loop III of Taiwan cobra cardiotoxin that is associated with the venom’s lethal potency. In dot blot analysis, several clones showed varying reactivities for NAV monovalent antivenin and lesser cross-reactions with BMV monovalent antivenin. The KSSLLRN-carrying phage occurred four times in selected clones and showed the strongest reactivity to NAV monovalent antivenin. Furthermore, the QDSLLPS-carrying phage also presented significant dot blot signal, indicating that the SLL sequence shared by these two clones may be a crucial antibody-binding site.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2009.07.097