Electrochemical biosensing based on universal affinity biocomposite platforms

• Published in: Biosensors & bioelectronics 2006-01, Vol.21 (7), p.1291-1301
• Main Authors: Zacco, E., Pividori, M.I., Alegret, S.
• Format: Article
• Language: English
• Subjects: (Strept)avidin; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensors; Biotechnology; Coated Materials, Biocompatible - analysis; Coated Materials, Biocompatible - chemistry; Electrochemical biosensing; Electrochemistry - instrumentation; Electrochemistry - methods; Epoxy Resins - analysis; Epoxy Resins - chemistry; Equipment Design; Equipment Failure Analysis; Fundamental and applied biological sciences. Psychology; Graphite - analysis; Graphite - chemistry; Graphite–epoxy biocomposite; Immunoassay - instrumentation; Immunoassay - methods; Manufactured Materials; Methods. Procedures. Technologies; Molecular Probe Techniques - instrumentation; Oligonucleotide Array Sequence Analysis - instrumentation; Oligonucleotide Array Sequence Analysis - methods; Protein Array Analysis - instrumentation; Protein Array Analysis - methods; Protein G; Protein Interaction Mapping - instrumentation; Protein Interaction Mapping - methods; Various methods and equipments
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2005.05.016

Rigid conducting biocomposites are versatile and effective transducing materials for the construction of a wide range of amperometric biosensors such as immunosensors, genosensors and enzymosensors, particularly if the transducer is bulk-modified with universal affinity biomolecules. The strept(avidin)–graphite–epoxy biocomposite could be considered as an universal immobilization platform whereon biotinylated DNAs, oligonucleotides, enzymes or antibodies can be captured by means of the highly affinity (strept)avidin–biotin reaction. Universal affinity biocomposite-based biosensors offer many potential advantages compared to more traditional electrochemical biosensors commonly based on a biologically surface-modified transducer. The integration of many materials into one matrix is their main advantage. As biological bulk-modified materials, the conducting biocomposites act not only as transducers, but also as reservoir for the biomaterial. After its use, the electrode surface can be renewed by a simple polishing procedure, establishing a clear advantage of these approaches relative to classical biosensors and other common biological assays. Moreover, the same material is useful for the analysis of many molecules whose determinations are based on genetic, enzymatic or immunological reactions. The different strategies for electrochemical genosensing, immunosensing and enzymosensing, all of them being dependent on the presence of a redox enzyme marker for the generation of the electrochemical signal, based on this universal affinity biocomposite platform are all presented and discussed.