Identification of a novel protein binding to hepatitis C virus core protein

Background:  Hepatitis C virus (HCV) core protein is a multi‐functional viral protein that interacts with several target proteins of both viral and cellular origin. Aim and Methods:  To gain insight into the mechanism of action of HCV core protein, we used a yeast two‐hybrid system to identify the c...

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Bibliographic Details
Published in:Journal of gastroenterology and hepatology 2009-07, Vol.24 (7), p.1300-1304
Main Authors: Chen, Yun-ru, Chen, Tian-yan, Zhang, Shu-lin, Lin, Shu-mei, Zhao, Ying-ren, Ye, Feng, Zhang, Xi, Shi, Lei, Dang, Shuang-suo, Liu, Min
Format: Article
Language:English
Subjects:
Amidohydrolases - genetics
Amidohydrolases - metabolism
Amino Acid Sequence
Animals
Binding Sites
Biological and medical sciences
Cercopithecus aethiops
core protein
COS Cells
Cytosol - enzymology
Gastroenterology. Liver. Pancreas. Abdomen
HCV
Hepacivirus - genetics
Hepacivirus - metabolism
Hepatitis C virus
Humans
Immunoprecipitation
Liver - enzymology
Medical sciences
Microscopy, Confocal
Microscopy, Fluorescence
Molecular Sequence Data
Protein Interaction Domains and Motifs
Protein Interaction Mapping
protein-protein interaction
Recombinant Fusion Proteins - metabolism
Transfection
Two-Hybrid System Techniques
Viral Core Proteins - genetics
Viral Core Proteins - metabolism
yeast two-hybrid
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Summary:Background:  Hepatitis C virus (HCV) core protein is a multi‐functional viral protein that interacts with several target proteins of both viral and cellular origin. Aim and Methods:  To gain insight into the mechanism of action of HCV core protein, we used a yeast two‐hybrid system to identify the core protein‐interacting cellular targets. Results:  A cDNA clone encoding an aspartoacylase was obtained, termed aspartoacylase 3 (ACY3). Interaction between ACY3 and HCV core protein was verified using a co‐immunoprecipitation assay in vitro, and a mammalian two‐hybrid system in vivo. Fluorescence microscopy showed green fluorescence protein‐fused ACY3 localized in the cytoplasm. Conclusion:  Our data suggest that ACY3 is an HCV core binding protein, which may play a role in the development of HCV‐associated diseases.
ISSN:0815-9319
1440-1746
DOI:10.1111/j.1440-1746.2009.05846.x