Labeled Schwann cell transplantation: Cell loss, host Schwann cell replacement, and strategies to enhance survival

Although transplanted Schwann cells (SCs) can promote axon regeneration and remyelination and improve recovery in models of spinal cord injury, little is known about their survival and how they interact with host tissue. Using labeled SCs from transgenic rats expressing human placental alkaline phos...

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Bibliographic Details
Published in:Glia 2006-02, Vol.53 (3), p.338-343
Main Authors: Hill, Caitlin E., Moon, Lawrence D.F., Wood, Patrick M., Bunge, Mary Bartlett
Format: Article
Language:English
Subjects:
alkaline phosphatase
Alkaline Phosphatase - biosynthesis
Alkaline Phosphatase - genetics
Animals
Apoptosis
Biological and medical sciences
Cell Survival
contusion
Cyclosporine - pharmacology
Female
Fundamental and applied biological sciences. Psychology
Humans
immunosuppression
Immunosuppressive Agents - pharmacology
Isolated neuron and nerve. Neuroglia
Necrosis
Nitroblue Tetrazolium
Placenta - enzymology
PLAP
rat
Rats
Rats, Inbred F344
Receptor, Nerve Growth Factor - metabolism
Schwann cell
Schwann Cells - physiology
Schwann Cells - transplantation
Spinal Cord Injuries - pathology
Spinal Cord Injuries - therapy
spinal cord injury
survival
transplantation
Vertebrates: nervous system and sense organs
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Summary:Although transplanted Schwann cells (SCs) can promote axon regeneration and remyelination and improve recovery in models of spinal cord injury, little is known about their survival and how they interact with host tissue. Using labeled SCs from transgenic rats expressing human placental alkaline phosphatase (PLAP), SC survival in a spinal cord contusion lesion was assessed. Few PLAP SCs survived at 2 weeks after acute transplantation. They died early due to necrosis and apoptosis. Delaying transplantation until 7 days after injury improved survival. A second wave of cell death occurred after surviving cells had integrated into the spinal cord. Survival of PLAP SCs was enhanced by immunosuppression with cyclosporin; delayed transplantation in conjunction with immunosuppression resulted in the best survival. In all cases, transplantation of SCs resulted in extensive infiltration of endogenous p75+ cells into the injury site, suggesting that endogenous SCs may play an important role in the repair observed after SC transplantation. © 2005 Wiley‐Liss, Inc.
ISSN:0894-1491
1098-1136
DOI:10.1002/glia.20287