The effect of leptin receptor deficiency and fasting on cannabinoid receptor 1 mRNA expression in the rat hypothalamus, brainstem and nodose ganglion

Despite ample evidence for the involvement of the endocannabinoid system in the control of appetite, food intake and energy balance, relatively little is known about the regulation of cannabinoid receptor 1 (CB 1R) expression in respect to leptin signalling and fasting. In the present study, we exam...

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Bibliographic Details
Published in:Neuroscience letters 2009-10, Vol.463 (2), p.125-129
Main Authors: Jelsing, Jacob, Larsen, Philip Just, Vrang, Niels
Format: Article
Language:English
Subjects:
Animals
Appetite - physiology
Biological and medical sciences
Brain Stem - metabolism
Brainstem
Cannabinoid receptor 1
Fasting
Food Deprivation
Fundamental and applied biological sciences. Psychology
Hypothalamus
Hypothalamus - metabolism
In Situ Hybridization
Leptin
Male
Medical sciences
Neuropharmacology
Nodose ganglion
Nodose Ganglion - metabolism
Obesity - metabolism
Pharmacology. Drug treatments
Psychodysleptics: hallucinogen
Psychology. Psychoanalysis. Psychiatry
Psychopharmacology
Rats
Rats, Sprague-Dawley
Rats, Zucker
Receptor, Cannabinoid, CB1 - biosynthesis
Receptor, Cannabinoid, CB1 - genetics
Receptors, Leptin - genetics
Receptors, Leptin - physiology
RNA, Messenger - biosynthesis
Signal Transduction
Vertebrates: nervous system and sense organs
Zucker rat
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Summary:Despite ample evidence for the involvement of the endocannabinoid system in the control of appetite, food intake and energy balance, relatively little is known about the regulation of cannabinoid receptor 1 (CB 1R) expression in respect to leptin signalling and fasting. In the present study, we examined CB 1R mRNA levels in lean (Fa/?) and obese (fa/fa) male Zucker rats under basal and food-restricted conditions. Using stereological sampling principles coupled with semi-quantitative radioactive in situ hybridization we provide semi-quantitative estimates of CB 1R mRNA expression in key appetite regulatory hypothalamic and brainstem areas, as well as in the nodose ganglia. Whereas no effect of fasting were determined on CB 1R mRNA levels in the paraventricular (PVN) and ventromedial hypothalamic (VMH) nucleus, in the brainstem dorsal vagal complex or nodose ganglion of lean Zucker rats, CB 1R mRNA levels were consistently elevated in obese Zucker rats pointing to a direct influence of disrupted leptin signalling on CB 1R mRNA regulation.
ISSN:0304-3940
1872-7972
DOI:10.1016/j.neulet.2009.07.011