Distinct cagA EPIYA Motifs are Associated with Ethnic Diversity in Malaysia and Singapore

Background:  In vitro studies have shown that the biologic activity of CagA is influenced by the number and class of EPIYA motifs present in its variable region as these motifs correspond to the CagA phosphorylation sites. It has been hypothesized that strains possessing specific combinations of the...

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Published in:Helicobacter (Cambridge, Mass.) Mass.), 2009-08, Vol.14 (4), p.256-263
Main Authors: Schmidt, Heather-Marie A., Goh, Khean-Lee, Fock, Kwong Ming, Hilmi, Ida, Dhamodaran, Subbiah, Forman, David, Mitchell, Hazel
Format: Article
Language:English
Subjects:
Adult
Aged
Amino Acid Motifs
Antigens, Bacterial - chemistry
Antigens, Bacterial - genetics
Antigens, Bacterial - metabolism
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
CagA
EPIYA motif
Female
gastric cancer
Helicobacter Infections - ethnology
Helicobacter Infections - microbiology
Helicobacter pylori
Helicobacter pylori - chemistry
Helicobacter pylori - genetics
Helicobacter pylori - isolation & purification
Humans
Malaysia
Malaysia - ethnology
Male
Middle Aged
Singapore
Singapore - ethnology
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Summary:Background:  In vitro studies have shown that the biologic activity of CagA is influenced by the number and class of EPIYA motifs present in its variable region as these motifs correspond to the CagA phosphorylation sites. It has been hypothesized that strains possessing specific combinations of these motifs may be responsible for gastric cancer development. This study investigated the prevalence of cagA and the EPIYA motifs with regard to number, class, and patterns in strains from the three major ethnic groups within the Malaysian and Singaporean populations in relation to disease development. Materials and methods:  Helicobacter pylori isolates from 49 Chinese, 43 Indian, and 14 Malay patients with functional dyspepsia (FD) and 21 gastric cancer (GC) cases were analyzed using polymerase chain reaction for the presence of cagA and the number, type, and pattern of EPIYA motifs. Additionally, the EPIYA motifs of 47 isolates were sequenced. Results:  All 126 isolates possessed cagA, with the majority encoding EPIYA‐A (97.6%) and all encoding EPIYA‐B. However, while the cagA of 93.0% of Indian FD isolates encoded EPIYA‐C as the third motif, 91.8% of Chinese FD isolates and 81.7% of Chinese GC isolates encoded EPIYA‐D (p 
ISSN:1083-4389
1523-5378
DOI:10.1111/j.1523-5378.2009.00684.x