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Distinct cagA EPIYA Motifs are Associated with Ethnic Diversity in Malaysia and Singapore
Background: In vitro studies have shown that the biologic activity of CagA is influenced by the number and class of EPIYA motifs present in its variable region as these motifs correspond to the CagA phosphorylation sites. It has been hypothesized that strains possessing specific combinations of the...
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| Published in: | Helicobacter (Cambridge, Mass.) Mass.), 2009-08, Vol.14 (4), p.256-263 |
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| creator | Schmidt, Heather-Marie A. Goh, Khean-Lee Fock, Kwong Ming Hilmi, Ida Dhamodaran, Subbiah Forman, David Mitchell, Hazel |
| description | Background: In vitro studies have shown that the biologic activity of CagA is influenced by the number and class of EPIYA motifs present in its variable region as these motifs correspond to the CagA phosphorylation sites. It has been hypothesized that strains possessing specific combinations of these motifs may be responsible for gastric cancer development. This study investigated the prevalence of cagA and the EPIYA motifs with regard to number, class, and patterns in strains from the three major ethnic groups within the Malaysian and Singaporean populations in relation to disease development.
Materials and methods: Helicobacter pylori isolates from 49 Chinese, 43 Indian, and 14 Malay patients with functional dyspepsia (FD) and 21 gastric cancer (GC) cases were analyzed using polymerase chain reaction for the presence of cagA and the number, type, and pattern of EPIYA motifs. Additionally, the EPIYA motifs of 47 isolates were sequenced.
Results: All 126 isolates possessed cagA, with the majority encoding EPIYA‐A (97.6%) and all encoding EPIYA‐B. However, while the cagA of 93.0% of Indian FD isolates encoded EPIYA‐C as the third motif, 91.8% of Chinese FD isolates and 81.7% of Chinese GC isolates encoded EPIYA‐D (p |
| doi_str_mv | 10.1111/j.1523-5378.2009.00684.x |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67586741</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20701414</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4354-afc333509e8a5d78d63c81116f453ea88ef9ac5a52ab2ddd70be00e92f7f48493</originalsourceid><addsrcrecordid>eNqNkdFP2zAQxq1p02Bs_wLy096SObGd2NJeKloKUtkGbAKerKtzAZc0KXY62v9-zlqxR3Yvd5K_707fz4TQjKVZrC-LNJM5TyQvVZozplPGCiXSzRty-PLwNs5M8URwpQ_IhxAWjDHJhX5PDjJdlCLL9SG5G7vQu9b21ML9iE5-nN-N6EXXuzpQ8EhHIXTWQY8VfXb9A530D62zdOx-ow-u31LX0gtoYBscUGgreu3ae1h1Hj-SdzU0AT_t-xH5dTr5eXKWzL5Pz09Gs8QKLkUCteWcS6ZRgaxKVRXcqpixqIXkCEphrcFKkDnM86qqSjZHxlDndVkLJTQ_Ip93e1e-e1pj6M3SBYtNAy1262CKUqoh7avCnJUsE5mIQrUTWt-F4LE2K--W4LcmY2bgbxZmwGwGzGbgb_7yN5toPd7fWM-XWP0z7oFHwded4Nk1uP3vxeZsMotDtCc7e_w13LzYwT_GnLyU5ubb1Ojb6-lsfHljrvgflB-iUw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20701414</pqid></control><display><type>article</type><title>Distinct cagA EPIYA Motifs are Associated with Ethnic Diversity in Malaysia and Singapore</title><source>Wiley</source><creator>Schmidt, Heather-Marie A. ; Goh, Khean-Lee ; Fock, Kwong Ming ; Hilmi, Ida ; Dhamodaran, Subbiah ; Forman, David ; Mitchell, Hazel</creator><creatorcontrib>Schmidt, Heather-Marie A. ; Goh, Khean-Lee ; Fock, Kwong Ming ; Hilmi, Ida ; Dhamodaran, Subbiah ; Forman, David ; Mitchell, Hazel</creatorcontrib><description>Background: In vitro studies have shown that the biologic activity of CagA is influenced by the number and class of EPIYA motifs present in its variable region as these motifs correspond to the CagA phosphorylation sites. It has been hypothesized that strains possessing specific combinations of these motifs may be responsible for gastric cancer development. This study investigated the prevalence of cagA and the EPIYA motifs with regard to number, class, and patterns in strains from the three major ethnic groups within the Malaysian and Singaporean populations in relation to disease development.
Materials and methods: Helicobacter pylori isolates from 49 Chinese, 43 Indian, and 14 Malay patients with functional dyspepsia (FD) and 21 gastric cancer (GC) cases were analyzed using polymerase chain reaction for the presence of cagA and the number, type, and pattern of EPIYA motifs. Additionally, the EPIYA motifs of 47 isolates were sequenced.
Results: All 126 isolates possessed cagA, with the majority encoding EPIYA‐A (97.6%) and all encoding EPIYA‐B. However, while the cagA of 93.0% of Indian FD isolates encoded EPIYA‐C as the third motif, 91.8% of Chinese FD isolates and 81.7% of Chinese GC isolates encoded EPIYA‐D (p < .001). Of Malay FD isolates, 61.5% and 38.5% possessed EPIYA‐C and EPIYA‐D, respectively. The majority of isolates possessed three EPIYA motifs; however, Indian isolates were significantly more likely to have four or more (p < .05).
Conclusion: Although, H. pylori strains with distinct cagA‐types are circulating within the primary ethnic groups resident in Malaysia and Singapore, these genotypes appear unassociated with the development of GC in the ethnic Chinese population. The phenomenon of distinct strains circulating within different ethnic groups, in combination with host and certain environmental factors, may help to explain the rates of GC development in Malaysia.</description><identifier>ISSN: 1083-4389</identifier><identifier>EISSN: 1523-5378</identifier><identifier>DOI: 10.1111/j.1523-5378.2009.00684.x</identifier><identifier>PMID: 19674129</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Aged ; Amino Acid Motifs ; Antigens, Bacterial - chemistry ; Antigens, Bacterial - genetics ; Antigens, Bacterial - metabolism ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; CagA ; EPIYA motif ; Female ; gastric cancer ; Helicobacter Infections - ethnology ; Helicobacter Infections - microbiology ; Helicobacter pylori ; Helicobacter pylori - chemistry ; Helicobacter pylori - genetics ; Helicobacter pylori - isolation & purification ; Humans ; Malaysia ; Malaysia - ethnology ; Male ; Middle Aged ; Singapore ; Singapore - ethnology</subject><ispartof>Helicobacter (Cambridge, Mass.), 2009-08, Vol.14 (4), p.256-263</ispartof><rights>2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4354-afc333509e8a5d78d63c81116f453ea88ef9ac5a52ab2ddd70be00e92f7f48493</citedby><cites>FETCH-LOGICAL-c4354-afc333509e8a5d78d63c81116f453ea88ef9ac5a52ab2ddd70be00e92f7f48493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19674129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schmidt, Heather-Marie A.</creatorcontrib><creatorcontrib>Goh, Khean-Lee</creatorcontrib><creatorcontrib>Fock, Kwong Ming</creatorcontrib><creatorcontrib>Hilmi, Ida</creatorcontrib><creatorcontrib>Dhamodaran, Subbiah</creatorcontrib><creatorcontrib>Forman, David</creatorcontrib><creatorcontrib>Mitchell, Hazel</creatorcontrib><title>Distinct cagA EPIYA Motifs are Associated with Ethnic Diversity in Malaysia and Singapore</title><title>Helicobacter (Cambridge, Mass.)</title><addtitle>Helicobacter</addtitle><description>Background: In vitro studies have shown that the biologic activity of CagA is influenced by the number and class of EPIYA motifs present in its variable region as these motifs correspond to the CagA phosphorylation sites. It has been hypothesized that strains possessing specific combinations of these motifs may be responsible for gastric cancer development. This study investigated the prevalence of cagA and the EPIYA motifs with regard to number, class, and patterns in strains from the three major ethnic groups within the Malaysian and Singaporean populations in relation to disease development.
Materials and methods: Helicobacter pylori isolates from 49 Chinese, 43 Indian, and 14 Malay patients with functional dyspepsia (FD) and 21 gastric cancer (GC) cases were analyzed using polymerase chain reaction for the presence of cagA and the number, type, and pattern of EPIYA motifs. Additionally, the EPIYA motifs of 47 isolates were sequenced.
Results: All 126 isolates possessed cagA, with the majority encoding EPIYA‐A (97.6%) and all encoding EPIYA‐B. However, while the cagA of 93.0% of Indian FD isolates encoded EPIYA‐C as the third motif, 91.8% of Chinese FD isolates and 81.7% of Chinese GC isolates encoded EPIYA‐D (p < .001). Of Malay FD isolates, 61.5% and 38.5% possessed EPIYA‐C and EPIYA‐D, respectively. The majority of isolates possessed three EPIYA motifs; however, Indian isolates were significantly more likely to have four or more (p < .05).
Conclusion: Although, H. pylori strains with distinct cagA‐types are circulating within the primary ethnic groups resident in Malaysia and Singapore, these genotypes appear unassociated with the development of GC in the ethnic Chinese population. The phenomenon of distinct strains circulating within different ethnic groups, in combination with host and certain environmental factors, may help to explain the rates of GC development in Malaysia.</description><subject>Adult</subject><subject>Aged</subject><subject>Amino Acid Motifs</subject><subject>Antigens, Bacterial - chemistry</subject><subject>Antigens, Bacterial - genetics</subject><subject>Antigens, Bacterial - metabolism</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>CagA</subject><subject>EPIYA motif</subject><subject>Female</subject><subject>gastric cancer</subject><subject>Helicobacter Infections - ethnology</subject><subject>Helicobacter Infections - microbiology</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - chemistry</subject><subject>Helicobacter pylori - genetics</subject><subject>Helicobacter pylori - isolation & purification</subject><subject>Humans</subject><subject>Malaysia</subject><subject>Malaysia - ethnology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Singapore</subject><subject>Singapore - ethnology</subject><issn>1083-4389</issn><issn>1523-5378</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqNkdFP2zAQxq1p02Bs_wLy096SObGd2NJeKloKUtkGbAKerKtzAZc0KXY62v9-zlqxR3Yvd5K_707fz4TQjKVZrC-LNJM5TyQvVZozplPGCiXSzRty-PLwNs5M8URwpQ_IhxAWjDHJhX5PDjJdlCLL9SG5G7vQu9b21ML9iE5-nN-N6EXXuzpQ8EhHIXTWQY8VfXb9A530D62zdOx-ow-u31LX0gtoYBscUGgreu3ae1h1Hj-SdzU0AT_t-xH5dTr5eXKWzL5Pz09Gs8QKLkUCteWcS6ZRgaxKVRXcqpixqIXkCEphrcFKkDnM86qqSjZHxlDndVkLJTQ_Ip93e1e-e1pj6M3SBYtNAy1262CKUqoh7avCnJUsE5mIQrUTWt-F4LE2K--W4LcmY2bgbxZmwGwGzGbgb_7yN5toPd7fWM-XWP0z7oFHwded4Nk1uP3vxeZsMotDtCc7e_w13LzYwT_GnLyU5ubb1Ojb6-lsfHljrvgflB-iUw</recordid><startdate>200908</startdate><enddate>200908</enddate><creator>Schmidt, Heather-Marie A.</creator><creator>Goh, Khean-Lee</creator><creator>Fock, Kwong Ming</creator><creator>Hilmi, Ida</creator><creator>Dhamodaran, Subbiah</creator><creator>Forman, David</creator><creator>Mitchell, Hazel</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>200908</creationdate><title>Distinct cagA EPIYA Motifs are Associated with Ethnic Diversity in Malaysia and Singapore</title><author>Schmidt, Heather-Marie A. ; Goh, Khean-Lee ; Fock, Kwong Ming ; Hilmi, Ida ; Dhamodaran, Subbiah ; Forman, David ; Mitchell, Hazel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4354-afc333509e8a5d78d63c81116f453ea88ef9ac5a52ab2ddd70be00e92f7f48493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Amino Acid Motifs</topic><topic>Antigens, Bacterial - chemistry</topic><topic>Antigens, Bacterial - genetics</topic><topic>Antigens, Bacterial - metabolism</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>CagA</topic><topic>EPIYA motif</topic><topic>Female</topic><topic>gastric cancer</topic><topic>Helicobacter Infections - ethnology</topic><topic>Helicobacter Infections - microbiology</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - chemistry</topic><topic>Helicobacter pylori - genetics</topic><topic>Helicobacter pylori - isolation & purification</topic><topic>Humans</topic><topic>Malaysia</topic><topic>Malaysia - ethnology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Singapore</topic><topic>Singapore - ethnology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schmidt, Heather-Marie A.</creatorcontrib><creatorcontrib>Goh, Khean-Lee</creatorcontrib><creatorcontrib>Fock, Kwong Ming</creatorcontrib><creatorcontrib>Hilmi, Ida</creatorcontrib><creatorcontrib>Dhamodaran, Subbiah</creatorcontrib><creatorcontrib>Forman, David</creatorcontrib><creatorcontrib>Mitchell, Hazel</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Helicobacter (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schmidt, Heather-Marie A.</au><au>Goh, Khean-Lee</au><au>Fock, Kwong Ming</au><au>Hilmi, Ida</au><au>Dhamodaran, Subbiah</au><au>Forman, David</au><au>Mitchell, Hazel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distinct cagA EPIYA Motifs are Associated with Ethnic Diversity in Malaysia and Singapore</atitle><jtitle>Helicobacter (Cambridge, Mass.)</jtitle><addtitle>Helicobacter</addtitle><date>2009-08</date><risdate>2009</risdate><volume>14</volume><issue>4</issue><spage>256</spage><epage>263</epage><pages>256-263</pages><issn>1083-4389</issn><eissn>1523-5378</eissn><abstract>Background: In vitro studies have shown that the biologic activity of CagA is influenced by the number and class of EPIYA motifs present in its variable region as these motifs correspond to the CagA phosphorylation sites. It has been hypothesized that strains possessing specific combinations of these motifs may be responsible for gastric cancer development. This study investigated the prevalence of cagA and the EPIYA motifs with regard to number, class, and patterns in strains from the three major ethnic groups within the Malaysian and Singaporean populations in relation to disease development.
Materials and methods: Helicobacter pylori isolates from 49 Chinese, 43 Indian, and 14 Malay patients with functional dyspepsia (FD) and 21 gastric cancer (GC) cases were analyzed using polymerase chain reaction for the presence of cagA and the number, type, and pattern of EPIYA motifs. Additionally, the EPIYA motifs of 47 isolates were sequenced.
Results: All 126 isolates possessed cagA, with the majority encoding EPIYA‐A (97.6%) and all encoding EPIYA‐B. However, while the cagA of 93.0% of Indian FD isolates encoded EPIYA‐C as the third motif, 91.8% of Chinese FD isolates and 81.7% of Chinese GC isolates encoded EPIYA‐D (p < .001). Of Malay FD isolates, 61.5% and 38.5% possessed EPIYA‐C and EPIYA‐D, respectively. The majority of isolates possessed three EPIYA motifs; however, Indian isolates were significantly more likely to have four or more (p < .05).
Conclusion: Although, H. pylori strains with distinct cagA‐types are circulating within the primary ethnic groups resident in Malaysia and Singapore, these genotypes appear unassociated with the development of GC in the ethnic Chinese population. The phenomenon of distinct strains circulating within different ethnic groups, in combination with host and certain environmental factors, may help to explain the rates of GC development in Malaysia.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19674129</pmid><doi>10.1111/j.1523-5378.2009.00684.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Aged Amino Acid Motifs Antigens, Bacterial - chemistry Antigens, Bacterial - genetics Antigens, Bacterial - metabolism Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism CagA EPIYA motif Female gastric cancer Helicobacter Infections - ethnology Helicobacter Infections - microbiology Helicobacter pylori Helicobacter pylori - chemistry Helicobacter pylori - genetics Helicobacter pylori - isolation & purification Humans Malaysia Malaysia - ethnology Male Middle Aged Singapore Singapore - ethnology |
| title | Distinct cagA EPIYA Motifs are Associated with Ethnic Diversity in Malaysia and Singapore |
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