Class 1 integron containing metallo β-lactamase gene blaIMP-1 in carbapenem-resistant Pseudomonas aeruginosa in Thailand

Abstract We investigated the genetic properties of two carbapenem-resistant Pseudomonas aeruginosa isolates collected from a regional hospital in the north of Thailand. Both isolates demonstrated high-level resistance to extended-spectrum cephalosporins and carbapenems. Detection of the MBL genes wa...

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Bibliographic Details
Published in:Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 2009-08, Vol.15 (4), p.257-261
Main Authors: Boonkerd, Nitsara, Pibalpakdi, Phannarai, Niumsup, Pannika R, Tiloklurs, Mujarin
Format: Article
Language:English
Subjects:
Amino Acid Sequence
beta-Lactamases - genetics
Carbapenems - pharmacology
Cephalosporins - pharmacology
Drug Resistance, Bacterial - genetics
Hematology, Oncology and Palliative Medicine
Humans
Infectious Diseases
Integrons - genetics
Medical Microbiology
Medicine
Medicine & Public Health
Molecular Epidemiology
Molecular Sequence Data
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - genetics
Pseudomonas Infections - epidemiology
Pseudomonas Infections - microbiology
Thailand - epidemiology
Virology
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Summary:Abstract We investigated the genetic properties of two carbapenem-resistant Pseudomonas aeruginosa isolates collected from a regional hospital in the north of Thailand. Both isolates demonstrated high-level resistance to extended-spectrum cephalosporins and carbapenems. Detection of the MBL genes was positive for blaIMP in both isolates. Pulsed field gel electrophoresis (PFGE) analysis showed that the two P. aeruginosa isolates were nonclonal. Molecular analysis of the blaIMP in isolate 837 showed the presence of a blaIMP-1 gene inserted in a class 1 integron. The blaIMP-1 was plasmid-mediated according to the transformation assay. This is the first case of the blaIMP-1 gene in carbapenem-resistant P. aeruginosa in Thailand, suggesting the further dissemination of this gene in Southeast Asia.
ISSN:1341-321X
1437-7780
DOI:10.1007/s10156-009-0684-X