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Class 1 integron containing metallo β-lactamase gene blaIMP-1 in carbapenem-resistant Pseudomonas aeruginosa in Thailand
Abstract We investigated the genetic properties of two carbapenem-resistant Pseudomonas aeruginosa isolates collected from a regional hospital in the north of Thailand. Both isolates demonstrated high-level resistance to extended-spectrum cephalosporins and carbapenems. Detection of the MBL genes wa...
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| Published in: | Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 2009-08, Vol.15 (4), p.257-261 |
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| Language: | English |
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| container_end_page | 261 |
| container_issue | 4 |
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| container_title | Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy |
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| creator | Boonkerd, Nitsara Pibalpakdi, Phannarai Niumsup, Pannika R Tiloklurs, Mujarin |
| description | Abstract We investigated the genetic properties of two carbapenem-resistant Pseudomonas aeruginosa isolates collected from a regional hospital in the north of Thailand. Both isolates demonstrated high-level resistance to extended-spectrum cephalosporins and carbapenems. Detection of the MBL genes was positive for blaIMP in both isolates. Pulsed field gel electrophoresis (PFGE) analysis showed that the two P. aeruginosa isolates were nonclonal. Molecular analysis of the blaIMP in isolate 837 showed the presence of a blaIMP-1 gene inserted in a class 1 integron. The blaIMP-1 was plasmid-mediated according to the transformation assay. This is the first case of the blaIMP-1 gene in carbapenem-resistant P. aeruginosa in Thailand, suggesting the further dissemination of this gene in Southeast Asia. |
| doi_str_mv | 10.1007/s10156-009-0684-X |
| format | article |
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Both isolates demonstrated high-level resistance to extended-spectrum cephalosporins and carbapenems. Detection of the MBL genes was positive for blaIMP in both isolates. Pulsed field gel electrophoresis (PFGE) analysis showed that the two P. aeruginosa isolates were nonclonal. Molecular analysis of the blaIMP in isolate 837 showed the presence of a blaIMP-1 gene inserted in a class 1 integron. The blaIMP-1 was plasmid-mediated according to the transformation assay. This is the first case of the blaIMP-1 gene in carbapenem-resistant P. aeruginosa in Thailand, suggesting the further dissemination of this gene in Southeast Asia.</description><identifier>ISSN: 1341-321X</identifier><identifier>EISSN: 1437-7780</identifier><identifier>DOI: 10.1007/s10156-009-0684-X</identifier><identifier>PMID: 19688247</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Amino Acid Sequence ; beta-Lactamases - genetics ; Carbapenems - pharmacology ; Cephalosporins - pharmacology ; Drug Resistance, Bacterial - genetics ; Hematology, Oncology and Palliative Medicine ; Humans ; Infectious Diseases ; Integrons - genetics ; Medical Microbiology ; Medicine ; Medicine & Public Health ; Molecular Epidemiology ; Molecular Sequence Data ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - genetics ; Pseudomonas Infections - epidemiology ; Pseudomonas Infections - microbiology ; Thailand - epidemiology ; Virology</subject><ispartof>Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 2009-08, Vol.15 (4), p.257-261</ispartof><rights>Japanese Society of Chemotherapy and the Japanese Association for Infectious Diseases</rights><rights>Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c266x-a88722d9a67b6365c69a1ad95fe4d664a78b786797115503f61d4071157a1b1e3</citedby><cites>FETCH-LOGICAL-c266x-a88722d9a67b6365c69a1ad95fe4d664a78b786797115503f61d4071157a1b1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19688247$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boonkerd, Nitsara</creatorcontrib><creatorcontrib>Pibalpakdi, Phannarai</creatorcontrib><creatorcontrib>Niumsup, Pannika R</creatorcontrib><creatorcontrib>Tiloklurs, Mujarin</creatorcontrib><title>Class 1 integron containing metallo β-lactamase gene blaIMP-1 in carbapenem-resistant Pseudomonas aeruginosa in Thailand</title><title>Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy</title><addtitle>J Infect Chemother</addtitle><addtitle>J Infect Chemother</addtitle><description>Abstract We investigated the genetic properties of two carbapenem-resistant Pseudomonas aeruginosa isolates collected from a regional hospital in the north of Thailand. Both isolates demonstrated high-level resistance to extended-spectrum cephalosporins and carbapenems. Detection of the MBL genes was positive for blaIMP in both isolates. Pulsed field gel electrophoresis (PFGE) analysis showed that the two P. aeruginosa isolates were nonclonal. Molecular analysis of the blaIMP in isolate 837 showed the presence of a blaIMP-1 gene inserted in a class 1 integron. The blaIMP-1 was plasmid-mediated according to the transformation assay. This is the first case of the blaIMP-1 gene in carbapenem-resistant P. aeruginosa in Thailand, suggesting the further dissemination of this gene in Southeast Asia.</description><subject>Amino Acid Sequence</subject><subject>beta-Lactamases - genetics</subject><subject>Carbapenems - pharmacology</subject><subject>Cephalosporins - pharmacology</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Hematology, Oncology and Palliative Medicine</subject><subject>Humans</subject><subject>Infectious Diseases</subject><subject>Integrons - genetics</subject><subject>Medical Microbiology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Molecular Epidemiology</subject><subject>Molecular Sequence Data</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - genetics</subject><subject>Pseudomonas Infections - epidemiology</subject><subject>Pseudomonas Infections - microbiology</subject><subject>Thailand - epidemiology</subject><subject>Virology</subject><issn>1341-321X</issn><issn>1437-7780</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp1kc2KFDEUhYMoztj6AG4kK3fRpH6S1EaQxp-BEQccoXfhVtXtssZU0uZWyfRr-SA-kym6YVau8nfO4eY7jL1U8o2S0rwlJVWthZSNkNpWYveIXaqqNMIYKx_nfVkpURZqd8GeEd1JqUxt7VN2oRptbVGZS3bceiDiio9hxiHFwLsYZhjDGAY-4QzeR_73j_DQzTABIR8wIG89XH25EauNd5BaOOTbSSSkkWYIM78hXPo4xQDEAdMyjCESrPLbHzB6CP1z9mQPnvDFed2w7x8_3G4_i-uvn662769FV2h9L8BaUxR9A9q0utR1pxtQ0Df1Hqte6wqMbY3VpjFK1bUs91r1lVwPBlSrsNyw16fcQ4q_FqTZTSN16PMMGBdyOjPRNmPbMHUSdikSJdy7QxonSEenpFt5uxNvl3m7lbfbZc-rc_jSTtg_OM6As6A4CSg_hQGTu4tLCvnD_029z6Z3JxNmLr_HbOp8bqQD_xOPSA8Jjgon3be157Vm2ZicUNjyH77poZ0</recordid><startdate>200908</startdate><enddate>200908</enddate><creator>Boonkerd, Nitsara</creator><creator>Pibalpakdi, Phannarai</creator><creator>Niumsup, Pannika R</creator><creator>Tiloklurs, Mujarin</creator><general>Springer Japan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200908</creationdate><title>Class 1 integron containing metallo β-lactamase gene blaIMP-1 in carbapenem-resistant Pseudomonas aeruginosa in Thailand</title><author>Boonkerd, Nitsara ; Pibalpakdi, Phannarai ; Niumsup, Pannika R ; Tiloklurs, Mujarin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c266x-a88722d9a67b6365c69a1ad95fe4d664a78b786797115503f61d4071157a1b1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amino Acid Sequence</topic><topic>beta-Lactamases - genetics</topic><topic>Carbapenems - pharmacology</topic><topic>Cephalosporins - pharmacology</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Hematology, Oncology and Palliative Medicine</topic><topic>Humans</topic><topic>Infectious Diseases</topic><topic>Integrons - genetics</topic><topic>Medical Microbiology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Molecular Epidemiology</topic><topic>Molecular Sequence Data</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - genetics</topic><topic>Pseudomonas Infections - epidemiology</topic><topic>Pseudomonas Infections - microbiology</topic><topic>Thailand - epidemiology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boonkerd, Nitsara</creatorcontrib><creatorcontrib>Pibalpakdi, Phannarai</creatorcontrib><creatorcontrib>Niumsup, Pannika R</creatorcontrib><creatorcontrib>Tiloklurs, Mujarin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boonkerd, Nitsara</au><au>Pibalpakdi, Phannarai</au><au>Niumsup, Pannika R</au><au>Tiloklurs, Mujarin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Class 1 integron containing metallo β-lactamase gene blaIMP-1 in carbapenem-resistant Pseudomonas aeruginosa in Thailand</atitle><jtitle>Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy</jtitle><stitle>J Infect Chemother</stitle><addtitle>J Infect Chemother</addtitle><date>2009-08</date><risdate>2009</risdate><volume>15</volume><issue>4</issue><spage>257</spage><epage>261</epage><pages>257-261</pages><issn>1341-321X</issn><eissn>1437-7780</eissn><abstract>Abstract We investigated the genetic properties of two carbapenem-resistant Pseudomonas aeruginosa isolates collected from a regional hospital in the north of Thailand. Both isolates demonstrated high-level resistance to extended-spectrum cephalosporins and carbapenems. Detection of the MBL genes was positive for blaIMP in both isolates. Pulsed field gel electrophoresis (PFGE) analysis showed that the two P. aeruginosa isolates were nonclonal. Molecular analysis of the blaIMP in isolate 837 showed the presence of a blaIMP-1 gene inserted in a class 1 integron. The blaIMP-1 was plasmid-mediated according to the transformation assay. This is the first case of the blaIMP-1 gene in carbapenem-resistant P. aeruginosa in Thailand, suggesting the further dissemination of this gene in Southeast Asia.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>19688247</pmid><doi>10.1007/s10156-009-0684-X</doi><tpages>5</tpages></addata></record> |
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| subjects | Amino Acid Sequence beta-Lactamases - genetics Carbapenems - pharmacology Cephalosporins - pharmacology Drug Resistance, Bacterial - genetics Hematology, Oncology and Palliative Medicine Humans Infectious Diseases Integrons - genetics Medical Microbiology Medicine Medicine & Public Health Molecular Epidemiology Molecular Sequence Data Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - genetics Pseudomonas Infections - epidemiology Pseudomonas Infections - microbiology Thailand - epidemiology Virology |
| title | Class 1 integron containing metallo β-lactamase gene blaIMP-1 in carbapenem-resistant Pseudomonas aeruginosa in Thailand |
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