MRI-compatible incubation chamber for cell culture experiments

Purpose To develop an incubation chamber that is compatible with MRI, while avoiding sources of error due to the experimental setup. Materials and Methods Two identical and gas‐tight chambers were constructed of Plexiglas™. The temperature and the CO2 concentration were adjustable. Temperature varia...

Full description

Saved in:
Bibliographic Details
Published in:Journal of magnetic resonance imaging 2006-01, Vol.23 (1), p.70-76
Main Authors: Schwenzer, Nina F., Grönewäller, Eckart F., Rodegerdts, Enno, Kehlbach, Rainer, Duda, Stephan H., Claussen, Claus D.
Format: Article
Language:English
Subjects:
adverse effects
Carbon Dioxide - analysis
cell culture
Cell Culture Techniques - instrumentation
Environment, Controlled
Equipment Design
Humidity
Hydrogen-Ion Concentration
in vitro experiments
incubation system
Incubators
magnetic fields
Magnetic Resonance Imaging
Phantoms, Imaging
Polymethyl Methacrylate
Statistics, Nonparametric
Temperature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose To develop an incubation chamber that is compatible with MRI, while avoiding sources of error due to the experimental setup. Materials and Methods Two identical and gas‐tight chambers were constructed of Plexiglas™. The temperature and the CO2 concentration were adjustable. Temperature variations within and between both chambers were measured. The pH values of the cell culture media were measured under different environmental settings. For each environment a colony formation test was carried out. The homogeneity of the magnetic field inside the chambers was estimated by phantom tests. Results The temperature variations within the chambers were
ISSN:1053-1807
1522-2586
DOI:10.1002/jmri.20475