Extraction of genomic DNA using a new amino silica monolithic column

A new amino silica monolithic column was developed for DNA extraction in a miniaturized format. The monolithic column was prepared in situ by polymerization of tetraethoxysilane (TEOS) and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane (AEAPMDMS). DNA was loaded in 50 mM tris(hydroxylmethyl)ami...

Full description

Saved in:
Bibliographic Details
Published in:Journal of separation science 2009-08, Vol.32 (15-16), p.2752-2758
Main Authors: Liu, Lijia, Yu, Shengbing, Yang, Shuixian, Zhou, Ping, Hu, Jiming, Zhang, Yibing
Format: Article
Language:English
Subjects:
Actins - genetics
Animals
Carps - genetics
Chromatography, Liquid - instrumentation
Chromatography, Liquid - methods
DNA - isolation & purification
DNA purification
Genome
Genomic DNA
Microfluidics
Microscopy, Electron, Scanning
Monolithic column
Silicon Dioxide - chemistry
Solid-phase extraction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c4678-5a15fa985ef9b66fd060764b5ca8a22c7b236298e187085bee6ddbbcd4d2af713
cites cdi_FETCH-LOGICAL-c4678-5a15fa985ef9b66fd060764b5ca8a22c7b236298e187085bee6ddbbcd4d2af713
container_end_page 2758
container_issue 15-16
container_start_page 2752
container_title Journal of separation science
container_volume 32
creator Liu, Lijia
Yu, Shengbing
Yang, Shuixian
Zhou, Ping
Hu, Jiming
Zhang, Yibing
description A new amino silica monolithic column was developed for DNA extraction in a miniaturized format. The monolithic column was prepared in situ by polymerization of tetraethoxysilane (TEOS) and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane (AEAPMDMS). DNA was loaded in 50 mM tris(hydroxylmethyl)aminomethane-EDTA buffer at pH 7.0 and eluted with 300 mM potassium phosphate solution at pH 10.0. Under optimal condition, a 6.0-cm monolithic column provided a capacity of 56 ng DNA with an extraction efficiency of 71 ± 5.2% (X ± RSD). When the amino silica monolithic column was applied to extract genomic DNA from the whole blood of crucian carp, an extraction efficiency of 52 ± 5.6% (X ± RSD) was obtained by three extractions. Since the chaotropic-based sample loading and organic solvent wash steps were avoided in this procedure, the purified DNA was suitable for downstream processes such as PCR. This amino silica monolithic column was demonstrated to allow rapid and efficient DNA purification in microscale.
doi_str_mv 10.1002/jssc.200900208
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67588884</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67588884</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4678-5a15fa985ef9b66fd060764b5ca8a22c7b236298e187085bee6ddbbcd4d2af713</originalsourceid><addsrcrecordid>eNqFkTtv2zAUhYkgRV7NmrHllE3uJSW-xsDOo4XhDm6QkaAoymEqiYkoIcm_DwMZbjffhZfAdw4uzkHogsCMANAfTzHaGQVQ6QPyAJ0QTlimclIc7nbgx-g0xicAIqSCI3RMFJOM5XCCFtdvQ2_s4EOHQ403rgutt3ixusJj9N0GG9y5V2xa3wUcfeOtwW3oQuOHx8TZ0Ixt9xV9qU0T3fn2PUP3N9d_5nfZ8vftz_nVMrMFFzJjhrDaKMlcrUrO6wo4CF6UzBppKLWipDmnSjoiBUhWOserqixtVVTU1ILkZ-hy8n3uw8vo4qBbH61rGtO5MEbNBZNpir1gXsgClNzvmDIFxpVK4GwCbR9i7F2tn3vfmv5dE9CfTejPJvSuiST4tnUey9ZV__Bt9AlQE_DqG_e-x07_Wq_n_5tnk9bHwb3ttKb_mzLIBdMPq1u9vCHqTtGVXib--8TXJmiz6X3U92sKJAfCBfB0zQcyeayZ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20805699</pqid></control><display><type>article</type><title>Extraction of genomic DNA using a new amino silica monolithic column</title><source>Wiley-Blackwell Read &amp; Publish Collection</source><creator>Liu, Lijia ; Yu, Shengbing ; Yang, Shuixian ; Zhou, Ping ; Hu, Jiming ; Zhang, Yibing</creator><creatorcontrib>Liu, Lijia ; Yu, Shengbing ; Yang, Shuixian ; Zhou, Ping ; Hu, Jiming ; Zhang, Yibing</creatorcontrib><description>A new amino silica monolithic column was developed for DNA extraction in a miniaturized format. The monolithic column was prepared in situ by polymerization of tetraethoxysilane (TEOS) and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane (AEAPMDMS). DNA was loaded in 50 mM tris(hydroxylmethyl)aminomethane-EDTA buffer at pH 7.0 and eluted with 300 mM potassium phosphate solution at pH 10.0. Under optimal condition, a 6.0-cm monolithic column provided a capacity of 56 ng DNA with an extraction efficiency of 71 ± 5.2% (X ± RSD). When the amino silica monolithic column was applied to extract genomic DNA from the whole blood of crucian carp, an extraction efficiency of 52 ± 5.6% (X ± RSD) was obtained by three extractions. Since the chaotropic-based sample loading and organic solvent wash steps were avoided in this procedure, the purified DNA was suitable for downstream processes such as PCR. This amino silica monolithic column was demonstrated to allow rapid and efficient DNA purification in microscale.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.200900208</identifier><identifier>PMID: 19585530</identifier><language>eng</language><publisher>Weinheim: Wiley-VCH Verlag</publisher><subject>Actins - genetics ; Animals ; Carps - genetics ; Chromatography, Liquid - instrumentation ; Chromatography, Liquid - methods ; DNA - isolation &amp; purification ; DNA purification ; Genome ; Genomic DNA ; Microfluidics ; Microscopy, Electron, Scanning ; Monolithic column ; Silicon Dioxide - chemistry ; Solid-phase extraction</subject><ispartof>Journal of separation science, 2009-08, Vol.32 (15-16), p.2752-2758</ispartof><rights>Copyright © 2009 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4678-5a15fa985ef9b66fd060764b5ca8a22c7b236298e187085bee6ddbbcd4d2af713</citedby><cites>FETCH-LOGICAL-c4678-5a15fa985ef9b66fd060764b5ca8a22c7b236298e187085bee6ddbbcd4d2af713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19585530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Lijia</creatorcontrib><creatorcontrib>Yu, Shengbing</creatorcontrib><creatorcontrib>Yang, Shuixian</creatorcontrib><creatorcontrib>Zhou, Ping</creatorcontrib><creatorcontrib>Hu, Jiming</creatorcontrib><creatorcontrib>Zhang, Yibing</creatorcontrib><title>Extraction of genomic DNA using a new amino silica monolithic column</title><title>Journal of separation science</title><addtitle>J. Sep. Science</addtitle><description>A new amino silica monolithic column was developed for DNA extraction in a miniaturized format. The monolithic column was prepared in situ by polymerization of tetraethoxysilane (TEOS) and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane (AEAPMDMS). DNA was loaded in 50 mM tris(hydroxylmethyl)aminomethane-EDTA buffer at pH 7.0 and eluted with 300 mM potassium phosphate solution at pH 10.0. Under optimal condition, a 6.0-cm monolithic column provided a capacity of 56 ng DNA with an extraction efficiency of 71 ± 5.2% (X ± RSD). When the amino silica monolithic column was applied to extract genomic DNA from the whole blood of crucian carp, an extraction efficiency of 52 ± 5.6% (X ± RSD) was obtained by three extractions. Since the chaotropic-based sample loading and organic solvent wash steps were avoided in this procedure, the purified DNA was suitable for downstream processes such as PCR. This amino silica monolithic column was demonstrated to allow rapid and efficient DNA purification in microscale.</description><subject>Actins - genetics</subject><subject>Animals</subject><subject>Carps - genetics</subject><subject>Chromatography, Liquid - instrumentation</subject><subject>Chromatography, Liquid - methods</subject><subject>DNA - isolation &amp; purification</subject><subject>DNA purification</subject><subject>Genome</subject><subject>Genomic DNA</subject><subject>Microfluidics</subject><subject>Microscopy, Electron, Scanning</subject><subject>Monolithic column</subject><subject>Silicon Dioxide - chemistry</subject><subject>Solid-phase extraction</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkTtv2zAUhYkgRV7NmrHllE3uJSW-xsDOo4XhDm6QkaAoymEqiYkoIcm_DwMZbjffhZfAdw4uzkHogsCMANAfTzHaGQVQ6QPyAJ0QTlimclIc7nbgx-g0xicAIqSCI3RMFJOM5XCCFtdvQ2_s4EOHQ403rgutt3ixusJj9N0GG9y5V2xa3wUcfeOtwW3oQuOHx8TZ0Ixt9xV9qU0T3fn2PUP3N9d_5nfZ8vftz_nVMrMFFzJjhrDaKMlcrUrO6wo4CF6UzBppKLWipDmnSjoiBUhWOserqixtVVTU1ILkZ-hy8n3uw8vo4qBbH61rGtO5MEbNBZNpir1gXsgClNzvmDIFxpVK4GwCbR9i7F2tn3vfmv5dE9CfTejPJvSuiST4tnUey9ZV__Bt9AlQE_DqG_e-x07_Wq_n_5tnk9bHwb3ttKb_mzLIBdMPq1u9vCHqTtGVXib--8TXJmiz6X3U92sKJAfCBfB0zQcyeayZ</recordid><startdate>200908</startdate><enddate>200908</enddate><creator>Liu, Lijia</creator><creator>Yu, Shengbing</creator><creator>Yang, Shuixian</creator><creator>Zhou, Ping</creator><creator>Hu, Jiming</creator><creator>Zhang, Yibing</creator><general>Wiley-VCH Verlag</general><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7U5</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>200908</creationdate><title>Extraction of genomic DNA using a new amino silica monolithic column</title><author>Liu, Lijia ; Yu, Shengbing ; Yang, Shuixian ; Zhou, Ping ; Hu, Jiming ; Zhang, Yibing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4678-5a15fa985ef9b66fd060764b5ca8a22c7b236298e187085bee6ddbbcd4d2af713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Actins - genetics</topic><topic>Animals</topic><topic>Carps - genetics</topic><topic>Chromatography, Liquid - instrumentation</topic><topic>Chromatography, Liquid - methods</topic><topic>DNA - isolation &amp; purification</topic><topic>DNA purification</topic><topic>Genome</topic><topic>Genomic DNA</topic><topic>Microfluidics</topic><topic>Microscopy, Electron, Scanning</topic><topic>Monolithic column</topic><topic>Silicon Dioxide - chemistry</topic><topic>Solid-phase extraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Lijia</creatorcontrib><creatorcontrib>Yu, Shengbing</creatorcontrib><creatorcontrib>Yang, Shuixian</creatorcontrib><creatorcontrib>Zhou, Ping</creatorcontrib><creatorcontrib>Hu, Jiming</creatorcontrib><creatorcontrib>Zhang, Yibing</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Lijia</au><au>Yu, Shengbing</au><au>Yang, Shuixian</au><au>Zhou, Ping</au><au>Hu, Jiming</au><au>Zhang, Yibing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extraction of genomic DNA using a new amino silica monolithic column</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. Science</addtitle><date>2009-08</date><risdate>2009</risdate><volume>32</volume><issue>15-16</issue><spage>2752</spage><epage>2758</epage><pages>2752-2758</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>A new amino silica monolithic column was developed for DNA extraction in a miniaturized format. The monolithic column was prepared in situ by polymerization of tetraethoxysilane (TEOS) and N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane (AEAPMDMS). DNA was loaded in 50 mM tris(hydroxylmethyl)aminomethane-EDTA buffer at pH 7.0 and eluted with 300 mM potassium phosphate solution at pH 10.0. Under optimal condition, a 6.0-cm monolithic column provided a capacity of 56 ng DNA with an extraction efficiency of 71 ± 5.2% (X ± RSD). When the amino silica monolithic column was applied to extract genomic DNA from the whole blood of crucian carp, an extraction efficiency of 52 ± 5.6% (X ± RSD) was obtained by three extractions. Since the chaotropic-based sample loading and organic solvent wash steps were avoided in this procedure, the purified DNA was suitable for downstream processes such as PCR. This amino silica monolithic column was demonstrated to allow rapid and efficient DNA purification in microscale.</abstract><cop>Weinheim</cop><pub>Wiley-VCH Verlag</pub><pmid>19585530</pmid><doi>10.1002/jssc.200900208</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1615-9306
ispartof Journal of separation science, 2009-08, Vol.32 (15-16), p.2752-2758
issn 1615-9306
1615-9314
language eng
recordid cdi_proquest_miscellaneous_67588884
source Wiley-Blackwell Read & Publish Collection
subjects Actins - genetics
Animals
Carps - genetics
Chromatography, Liquid - instrumentation
Chromatography, Liquid - methods
DNA - isolation & purification
DNA purification
Genome
Genomic DNA
Microfluidics
Microscopy, Electron, Scanning
Monolithic column
Silicon Dioxide - chemistry
Solid-phase extraction
title Extraction of genomic DNA using a new amino silica monolithic column
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T22%3A00%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Extraction%20of%20genomic%20DNA%20using%20a%20new%20amino%20silica%20monolithic%20column&rft.jtitle=Journal%20of%20separation%20science&rft.au=Liu,%20Lijia&rft.date=2009-08&rft.volume=32&rft.issue=15-16&rft.spage=2752&rft.epage=2758&rft.pages=2752-2758&rft.issn=1615-9306&rft.eissn=1615-9314&rft_id=info:doi/10.1002/jssc.200900208&rft_dat=%3Cproquest_cross%3E67588884%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4678-5a15fa985ef9b66fd060764b5ca8a22c7b236298e187085bee6ddbbcd4d2af713%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=20805699&rft_id=info:pmid/19585530&rfr_iscdi=true