TRPV6 potentiates calcium-dependent cell proliferation

The Ca 2+ homeostasis within cells controls a diversity of cellular processes including gene transcription, proliferation and apoptosis. Perturbance of Ca 2+ signaling may induce deregulation of cell proliferation and suppression of cell death providing the basis for cancer development. In human pro...

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Bibliographic Details
Published in:Cell calcium (Edinburgh) 2006-02, Vol.39 (2), p.163-173
Main Authors: Schwarz, Eva C., Wissenbach, Ulrich, Niemeyer, Barbara A., Strauß, Bettina, Philipp, Stephan E., Flockerzi, Veit, Hoth, Markus
Format: Article
Language:English
Subjects:
Anilides - pharmacology
Calcium channel
Calcium Channels - genetics
Calcium Channels - metabolism
Calcium homeostasis
Calcium Signaling - drug effects
Calcium Signaling - physiology
Cell Line
Cell Proliferation - drug effects
Cell Survival
Econazole - pharmacology
Gene Expression
Humans
Male
Proliferation
Prostate cancer
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Thiadiazoles - pharmacology
Transfection
TRP channel
TRPV Cation Channels - genetics
TRPV Cation Channels - metabolism
TRPV6
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Summary:The Ca 2+ homeostasis within cells controls a diversity of cellular processes including gene transcription, proliferation and apoptosis. Perturbance of Ca 2+ signaling may induce deregulation of cell proliferation and suppression of cell death providing the basis for cancer development. In human prostate cancer, a correlation between the mRNA expression of the Ca 2+ channel TRPV6 and the staging of the cancer has been described. We have analyzed the influence of TRPV6 on cell proliferation within HEK-293 cells. We show that TRPV6 increases cell proliferation of HEK-293 cells in a Ca 2+ dependent manner. The increased proliferation correlates with slightly increased intracellular Ca 2+ levels without interfering with the intrinsic Ca 2+ dependence of HEK-293 cell proliferation. Low doses of econazole inhibit both, TRPV6 dependent Ca 2+ signals and cell proliferation while BTP2, a potent inhibitor of Ca 2+ signals and cell proliferation in T-cells, neither influences TRPV6 dependent Ca 2+ signals nor cell proliferation of HEK-293 cells. Our data demonstrate that TRPV6 increases the rate of Ca 2+ dependent cell proliferation which is a prerequisite for its potential role in tumor progression.
ISSN:0143-4160
1532-1991
DOI:10.1016/j.ceca.2005.10.006