Neutralizing antibody levels for protection against betanodavirus infection in sevenband grouper, Epinephelus septemfasciatus (Thunberg), immunized with an inactivated virus vaccine

An inactivated betanodavirus, red-spotted grouper nervous necrosis virus (RGNNV), is a vaccine candidate for viral nervous necrosis (VNN). The present study was conducted to examine inoculation doses of the vaccine and neutralizing antibody titre levels to protect fish against VNN. Young sevenband g...

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Bibliographic Details
Published in:Journal of fish diseases 2009-09, Vol.32 (9), p.767-775
Main Authors: Yamashita, H, Mori, K, Kuroda, A, Nakai, T
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - blood
antibody
Bass - immunology
Betanodavirus
Epinephelus
Fish Diseases - immunology
Fish Diseases - prevention & control
Fish Diseases - virology
immunization
Nodaviridae - immunology
RNA Virus Infections - immunology
RNA Virus Infections - prevention & control
RNA Virus Infections - veterinary
RNA Virus Infections - virology
Time Factors
vaccine
Vaccines, Inactivated - immunology
Viral Vaccines - immunology
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Summary:An inactivated betanodavirus, red-spotted grouper nervous necrosis virus (RGNNV), is a vaccine candidate for viral nervous necrosis (VNN). The present study was conducted to examine inoculation doses of the vaccine and neutralizing antibody titre levels to protect fish against VNN. Young sevenband grouper, Epinephelus septemfasciatus, averaging 25.4 g, were immunized at 25 °C water temperature by a single intraperitoneal injection of formalin-inactivated RGNNV. Fish immunized at vaccine doses of 10⁸.⁵, 10⁸.⁰, 10⁷.⁵, 10⁷.⁰ and 10⁶.⁵ TCID₅₀ per fish produced antibodies at mean titres of 1:907, 1:511, 1:259, 1:197 and 1:96, respectively, at 20 days post-immunization (p.i.). Neutralizing antibodies were not detected in any control fish (titre
ISSN:0140-7775
1365-2761
DOI:10.1111/j.1365-2761.2009.01054.x