Induction of phenolic compounds in Hypericum perforatum L. cells by Colletotrichum gloeosporioides elicitation

• Published in: Phytochemistry (Oxford) 2006, Vol.67 (2), p.149-155
• Main Authors: Conceição, Luis F.R., Ferreres, Federico, Tavares, Rui M., Dias, Alberto C.P.
• Format: Article
• Language: English
• Subjects: Acetates - pharmacology; Biological and medical sciences; Biotic stress; cell suspension culture; Cells, Cultured; Chemical constitution; Chromatography, High Pressure Liquid; Colletotrichum - chemistry; Colletotrichum - metabolism; Colletotrichum gloeosporioides; cultured cells; Cyclopentanes - pharmacology; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Glomerella cingulata; HPLC–DAD–MS/MS; Hypericum - chemistry; Hypericum - drug effects; Hypericum - metabolism; Hypericum perforatum; Methyl-jasmonate; Oxylipins; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; phenolic compounds; Phenolics; Phenols - chemistry; Phenols - metabolism; Phytopathology. Animal pests. Plant and forest protection; plant biochemistry; plant pathogenic fungi; Plant physiology and development; Salicylic acid; Salicylic Acid - pharmacology; signal transduction; Species Specificity; Xanthones; Xanthones - chemistry; Xanthones - metabolism
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2005.10.017

Hypericum perforatum (HP) cultures were used to study the plant defence against the fungal pathogen Colletotrichum gloeosporioides (CG). A significant increase in xanthone accumulation was observed in HP cells elicited with CG. Priming of HP cells with methyl-jasmonate or salicylic acid prior to CG elicitation led to a xanthone burst. Changes in phenolic metabolism after elicitation with Colletotrichum gloeosporioides (CG) has been studied in Hypericum perforatum L. (HP) cell suspension cultures. Soluble phenolics were analysed by HPLC–DAD and HPLC–DAD–MS/MS. HP cultures elicited with the CG elicitor showed a significant increase in xanthone accumulation. Xanthone accumulation increased twelve fold when the cells were primed with methyl-jasmonate (MeJ) or salicylic acid (SA), before elicitation. HP cultures exposed only to MeJ produced a set of flavonoids, the flavones which represent a substantial part (approx. 40%) of the total flavonoids accumulated in these cells. The possible importance of xanthones as a component of defence mechanism of HP against biotic stress is discussed.