Production and purification of recombinant somatolactin β and its effects on melanosome aggregation in zebrafish

A second form of somatolactin, somatolactin beta (SLβ), was recently discovered in zebrafish ( Danio rerio). This novel subtype of somatolactin is distantly related to somatolactin alpha (SLα) found in teleost species and is produced in a different region of the pituitary. To date, no physiological...

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Bibliographic Details
Published in:General and comparative endocrinology 2006-01, Vol.145 (2), p.182-187
Main Authors: Nguyen, Nhu, Sugimoto, Masazumi, Zhu, Yong
Format: Article
Language:English
Subjects:
Animals
Cloning, Molecular
Danio rerio
DNA, Complementary - metabolism
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Fish Proteins - biosynthesis
Fish Proteins - genetics
Fish Proteins - pharmacology
Glycoproteins - biosynthesis
Glycoproteins - genetics
Glycoproteins - pharmacology
Growth hormone
Melanocytes - physiology
Melanocytes - ultrastructure
Melanophores
Melanosome
Melanosomes - drug effects
Melanosomes - physiology
Pituitary Gland - cytology
Pituitary Gland - metabolism
Pituitary Hormones - biosynthesis
Pituitary Hormones - genetics
Pituitary Hormones - pharmacology
Prolactin
Recombinant Proteins - biosynthesis
Recombinant Proteins - isolation & purification
Recombinant Proteins - pharmacology
Skin - drug effects
Skin - ultrastructure
Somatolactin α
Somatolactin β
Teleostei
Zebrafish
Zebrafish - anatomy & histology
Zebrafish - physiology
Zebrafish Proteins - biosynthesis
Zebrafish Proteins - genetics
Zebrafish Proteins - pharmacology
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Description
Summary:A second form of somatolactin, somatolactin beta (SLβ), was recently discovered in zebrafish ( Danio rerio). This novel subtype of somatolactin is distantly related to somatolactin alpha (SLα) found in teleost species and is produced in a different region of the pituitary. To date, no physiological study of SLβ has been reported. In order to study the physiological functions of SLβ, recombinant SLβ protein has been produced and purified. The cDNA of zebrafish SLβ was cloned into a pET100 bacteria expression vector and His-tagged fusion proteins were produced in BL21 (DE3) Escherichia coli cells. The majority of recombinant somatolactins produced by E. coli were isolated in inclusion bodies although a small percentage of recombinant proteins (
ISSN:0016-6480
1095-6840
DOI:10.1016/j.ygcen.2005.09.015