The effects of repeated corticosteroid administration on the neurogenesis in the neonatal rat

The purpose of this study is to clarify the effects on the brain including neurogenesis pretreated with repeated doses of dexamethasone in the neonatal rat. The 4-day-old Sprague Dawley rats were pretreated with 4 different regimens, namely, single administration of dexamethasone, 2-dose administrat...

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Bibliographic Details
Published in:American journal of obstetrics and gynecology 2006, Vol.194 (1), p.231-238
Main Authors: Kanagawa, Takeshi, Tomimatsu, Takuji, Hayashi, Shusaku, Shioji, Mitsunori, Fukuda, Hiromitsu, Shimoya, Koichiro, Murata, Yuji
Format: Article
Language:English
Subjects:
Aging - metabolism
Animals
Animals, Newborn - growth & development
Biological and medical sciences
Body Weight - drug effects
Brain - cytology
Brain - drug effects
Brain - growth & development
Brain - metabolism
Bromodeoxyuridine
Bromodeoxyuridine - pharmacokinetics
Cell Proliferation - drug effects
Dexamethasone - administration & dosage
Dexamethasone - pharmacology
Dose-Response Relationship, Drug
Drug Administration Schedule
Gynecology. Andrology. Obstetrics
Immunohistochemistry
Medical sciences
Mitosis
Neonatal rat
Nerve Tissue Proteins - metabolism
Neurogenesis
Nuclear Proteins - metabolism
Phenotype
Rats
Rats, Sprague-Dawley
Repeated corticosteroid
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Summary:The purpose of this study is to clarify the effects on the brain including neurogenesis pretreated with repeated doses of dexamethasone in the neonatal rat. The 4-day-old Sprague Dawley rats were pretreated with 4 different regimens, namely, single administration of dexamethasone, 2-dose administration, 3-dose administration, and saline administration as a control. Concurrently, bromodeoxyuridine (BrdU), which was incorporated into the dividing cells, was administered. We examined body weight, brain weight, and the number of BrdU-labeled cells in the subventricular zone (SVZ), the subgranular zone (SGZ), and the cortex. Both the body and brain weight of the rats pretreated with dexamethasone were significantly decreased compared with those given saline. Quantitative analysis of BrdU-labeled cells revealed the significant dose-dependent decreases in the SVZ, the SGZ, and the cortex with the dexamethasone treatment. We concluded that the decreases in neurogenesis caused by repeated antenatal corticosteroid therapy might result in the adverse effects on the size of the head at birth.
ISSN:0002-9378
1097-6868
DOI:10.1016/j.ajog.2005.06.015