Nucleotide sequence and embryonic expression of quail and duck Sox9 genes

Sox9 is a member of the Sry-type HMG-box (Sox) gene family. It encodes a transcription factor and is thought to be important for sexual differentiation in chicken. In the present study we have isolated Sox9 cDNAs from quail and duck, and examined the expression patterns of the corresponding genes in...

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Bibliographic Details
Published in:General and comparative endocrinology 2006-01, Vol.145 (2), p.208-213
Main Authors: Takada, Shuji, Ota, Jun, Kansaku, Norio, Yamashita, Hideji, Izumi, Tokukazu, Ishikawa, Madoka, Wada, Tomoaki, Kaneda, Ruri, Choi, Young Lim, Koinuma, Koji, Fujiwara, Shin-ichiro, Aoki, Hirotaka, Kisanuki, Hiroyuki, Yamashita, Yoshihiro, Mano, Hiroyuki
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Avian Proteins - genetics
Avian Proteins - metabolism
Base Sequence
Duck
Ducks - embryology
Ducks - genetics
Ducks - metabolism
Embryo, Nonmammalian - metabolism
Female
Gonads - anatomy & histology
Gonads - metabolism
High Mobility Group Proteins - genetics
High Mobility Group Proteins - metabolism
In Situ Hybridization
Male
Molecular Sequence Data
Polymerase Chain Reaction - methods
Quail
Quail - embryology
Quail - genetics
Quail - metabolism
Sequence Alignment
Sex determination
Sex Determination Analysis
Sex Differentiation - genetics
Sexing
Sox9
SOX9 Transcription Factor
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Sox9 is a member of the Sry-type HMG-box (Sox) gene family. It encodes a transcription factor and is thought to be important for sexual differentiation in chicken. In the present study we have isolated Sox9 cDNAs from quail and duck, and examined the expression patterns of the corresponding genes in early embryonic gonads by whole-mount in situ hybridization. We developed a polymerase chain reaction-based protocol to identify the sex of quail and duck embryos before its morphological manifestation. Sox9 expression was first detected on days 5 and 7 in the gonads of male quail and duck embryos, respectively, and was not apparent in female gonads at these stages. These expression patterns are similar to that of chicken Sox9. Our results thus suggest that the expression of quail and duck Sox9 is associated with testis differentiation.
ISSN:0016-6480
1095-6840
DOI:10.1016/j.ygcen.2005.08.009