Hsp70 prevents disuse muscle atrophy in senescent rats

This study determined the effects of heat shock protein 70 (Hsp70) overexpression on disuse muscle atrophy in senescent rats. Solei of young and senescent rats were co-injected with Hsp70 plus a nuclear factor kappa B (NF-κΒ) reporter plasmid. After 4 days, the hind limbs of half the young and senes...

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Bibliographic Details
Published in:Biogerontology (Dordrecht) 2009-10, Vol.10 (5), p.605-611
Main Authors: Dodd, Stephen, Hain, Brian, Judge, Andrew
Format: Article
Language:English
Subjects:
Aging
Aging - physiology
Animals
Biological and medical sciences
Biomedical and Life Sciences
Cell Biology
Development. Metamorphosis. Moult. Ageing
Developmental Biology
Fundamental and applied biological sciences. Psychology
Geriatrics/Gerontology
Heat shock proteins
HSP70 Heat-Shock Proteins - genetics
HSP70 Heat-Shock Proteins - metabolism
Humans
I-kappa B Proteins - metabolism
Immobilization
Life Sciences
Male
Muscle, Skeletal - anatomy & histology
Muscle, Skeletal - metabolism
Muscle, Skeletal - pathology
Muscular Disorders, Atrophic - genetics
Muscular Disorders, Atrophic - metabolism
Musculoskeletal system
NF-KappaB Inhibitor alpha
Older people
Plasmids
Rats
Rats, Inbred F344
Reporters
Research Article
Sarcopenia
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:This study determined the effects of heat shock protein 70 (Hsp70) overexpression on disuse muscle atrophy in senescent rats. Solei of young and senescent rats were co-injected with Hsp70 plus a nuclear factor kappa B (NF-κΒ) reporter plasmid. After 4 days, the hind limbs of half the young and senescent rats were immobilized for 6 days with the remainder serving as weight bearing controls. Hsp70 protein levels and cross-sectional area decreased in both groups (~20%) after immobilization. Atrophy was prevented in those fibers overexpressing Hsp70. NF-κΒ activity increased in the soleus of both young (three-fold) and senescent (five-fold) animals after immobilization and was prevented by Hsp70 overexpression. Inhibitor of κΒ decreased in young (~30%) and senescent (~10%) animals with immobilization and returned to normal with Hsp70. Heat shock protein 70 overexpression prevents disuse atrophy in senescent rats, possibly through suppression of the NF-κB pathway.
ISSN:1389-5729
1573-6768
DOI:10.1007/s10522-008-9203-1