Up-regulation of metallothionein isoforms in keloid keratinocytes

Keloids are proliferative growths of dermal collagen, usually resulting from excessive tissue response during wound healing. There is evidence that keratinocytes may promote keloidogenesis via epithelial-mesenchymal interactions. Metallothioneins (MTs) are known to be involved in the fundamental cel...

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Bibliographic Details
Published in:International journal of molecular medicine 2006-02, Vol.17 (2), p.385-389
Main Authors: Lim, D, Phan, T, Yip, G, Bay, B
Format: Article
Language:English
Subjects:
Cells, Cultured
Humans
Keloid - genetics
Keloid - pathology
Keratinocytes - metabolism
Keratinocytes - ultrastructure
Metallothionein - genetics
Microscopy, Electron, Transmission
Protein Isoforms - genetics
RNA, Messenger - genetics
Up-Regulation - genetics
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Summary:Keloids are proliferative growths of dermal collagen, usually resulting from excessive tissue response during wound healing. There is evidence that keratinocytes may promote keloidogenesis via epithelial-mesenchymal interactions. Metallothioneins (MTs) are known to be involved in the fundamental cellular processes of growth and apoptosis. In this study, we evaluated the expression of MT isoforms in normal and keloid keratinocytes. The expression patterns of ten functional MT isoforms were assessed using real-time RT-PCR in primary cultures of normal and keloid keratinocytes. The MT-2A isoform was the most abundant MT isoform in both normal and keloid keratinocytes while the MT-1B isoform was absent. There was a significant increase in the mRNA expression of four MT isoforms, viz. MT-1A, 1E, 1F and 2A in keloid keratinocytes as compared to normal keratinocytes. Up-regulation of MT-1A, 1E, 1F and 2A isoforms may play a part in the development of keloids by paracrine signaling.
ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.17.2.385