Modulation of Neutrophil Apoptosis in Plasma of Patients after Orthognathic Surgery

• Published in: The Journal of surgical research 2006, Vol.130 (1), p.110-118
• Main Authors: Suzuki, Rikako, Iwase, Masayasu, Miyaoka, Ken-ichi, Kondo, Gen, Watanabe, Hitoshi, Ohashi, Masaru, Nagumo, Masao
• Format: Article
• Language: English
• Subjects: Adult; Antibodies - immunology; apoptosis; Apoptosis - drug effects; Apoptosis - immunology; Biological and medical sciences; C-Reactive Protein - metabolism; Flow Cytometry; General aspects; Humans; inflammatory response; interleukin-8; Interleukin-8 - blood; Interleukin-8 - immunology; Interleukin-8 - pharmacology; Leukocyte Count; Medical sciences; neutrophils; Neutrophils - cytology; Neutrophils - drug effects; Neutrophils - metabolism; Oral Surgical Procedures; Postoperative Period; Receptors, Interleukin-8A - metabolism; Receptors, Interleukin-8B - metabolism; Stress, Physiological - immunology; surgical stress
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2005.08.001

Human neutrophils undergo rapid apoptosis during in vitro culture. The aim of this study was to investigate the role of interleukin-8 (IL-8) on neutrophil apoptosis in surgery-induced inflammation. Blood samples were drawn from 21 patients with mandibular prognathism 2 days before, and 1 and 5 days after orthognathic surgery. The IL-8 levels in the separated plasma were measured using an ELISA kit. The expression of two receptors for IL-8, CXCR1, and CXCR2, and their role in neutrophil apoptosis was evaluated using a flow cytometer. The IL-8 levels in the plasma were correlated with acute inflammatory markers, such as peripheral blood neutrophil counts and C-reactive protein levels. Both IL-8 receptors were markedly raised in patient-derived neutrophils 1 day post-operatively. Recombinant IL-8 (0–100 ng/ml) suppressed apoptosis in fresh-isolated neutrophils from healthy donors dose-dependently. Neutrophil apoptosis 1 day post-operatively was slightly accelerated in the presence of fetal bovine serum compared to the value 2 days pre-operatively and 5 days post-operatively. In contrast, in the presence of autogenous plasma, neutrophil apoptosis was significantly suppressed 1 day post-operatively compared to the value 2 days pre-operatively and 5 days post-operatively. Moreover, the anti-apoptotic effect of plasma on neutrophil apoptosis was partially decreased by the addition of anti-IL-8 neutralizing antibody. These results suggest that circulating neutrophils are susceptible to augmentation by IL-8 through the reinforcement of IL-8 receptors in acute inflammatory conditions. Furthermore, IL-8 may, in part, contribute to the regulation of neutrophil survival during the inflammatory response.