Downsizing of Enzymes by Chemical Methods: Arginine Mimics with Low pKa Values Increase the Rates of Hydrolysis of RNA Model Compounds

A talented mimic: Large rate enhancements were observed in the catalysis of reactions of RNA models when two arginine residues and two histidine residues were replaced with residues with a guanidiniocarbonyl pyrrole (Gcp) side chain (see structure) in a folded polypeptide catalyst. Gcp fulfilled the...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2009-08, Vol.48 (36), p.6722-6725
Main Authors: Lindgren, N. Johan V, Geiger, Lars, Razkin, Jesus, Schmuck, Carsten, Baltzer, Lars
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arginine - chemistry
Catalytic Domain
de novo design
Enzymes - chemistry
homogeneous catalysis
Hydrogen-Ion Concentration
Hydrolysis
Models, Biological
Molecular Mimicry
Molecular Sequence Data
Molecular Structure
peptides
phosphodiesters
RNA - chemistry
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A talented mimic: Large rate enhancements were observed in the catalysis of reactions of RNA models when two arginine residues and two histidine residues were replaced with residues with a guanidiniocarbonyl pyrrole (Gcp) side chain (see structure) in a folded polypeptide catalyst. Gcp fulfilled the functions of, and even outperformed, both Arg and His.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200900595