Anti-inflammatory Effect of Propolis through Inhibition of Nitric Oxide Production on Carrageenin-Induced Mouse Paw Edema

• Published in: Biological & Pharmaceutical Bulletin 2006, Vol.29(1), pp.96-99
• Main Authors: Tan-no, Koichi, Nakajima, Takeharu, Shoji, Takehiro, Nakagawasai, Osamu, Niijima, Fukie, Ishikawa, Masaaki, Endo, Yasuo, Sato, Takumi, Satoh, Susumu, Tadano, Takeshi
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents, Non-Steroidal; Area Under Curve; Arginine - pharmacology; Carrageenan; carrageenin; diclofenac; Diclofenac - pharmacology; Edema - chemically induced; Edema - metabolism; Enzyme Inhibitors - pharmacology; L-NG-nitro arginine methyl ester; Male; Mice; mouse; NG-Nitroarginine Methyl Ester - pharmacology; Nitric Oxide - biosynthesis; Nitric Oxide Synthase Type I - antagonists & inhibitors; paw edema; propolis; Propolis - pharmacology
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.29.96

The anti-inflammatory effect of propolis was compared with that of diclofenac, a non-steroidal anti-inflammatory drug, and L-NG-nitro arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, using carrageenin-induced mouse paw edema. When administered 10 min prior to carrageenin injection, propolis (1 : 1000, 1 : 100, p.o.), diclofenac (12.5, 50 mg/kg, p.o.) and L-NAME (10, 100 mg/kg, s.c.) showed a significant anti-inflammatory effect. The anti-inflammatory effects of propolis and L-NAME were significantly inhibited by L-arginine, a precursor of nitric oxide, but not by D-arginine. In contrast, the anti-inflammatory effect produced by diclofenac was not inhibited by either D-arginine or L-arginine. These results indicate that the anti-inflammatory effect of propolis on mouse paw edema acts via the inhibition of nitric oxide production, similar to that of L-NAME but not diclofenac.