Inhibition of Melanogenic Activity by 4,4′-Dihydroxybiphenyl in Melanoma Cells

In our previous study, we showed that 4,4′-dihydroxybiphenyl (44′-BP) reduced melanin content via the inhibition of tyrosinase. In the current study, we utilized 44′-BP treated B16 melanoma cells (B16 cells) to measure several key cellular parameters known to be involved in melanogenic activity. Inc...

Full description

Saved in:
Bibliographic Details
Published in:Biological & Pharmaceutical Bulletin 2006, Vol.29(1), pp.14-16
Main Authors: No, Jae Kyung, Kim, You Jung, Lee, Jun Sik, Chung, Hae Young
Format: Article
Language:English
Subjects:
4,4′-dihydroxybiphenyl
Biphenyl Compounds - pharmacology
Blotting, Western
Cell Line, Tumor
cyclic AMP
Cyclic AMP - metabolism
Cyclic AMP-Dependent Protein Kinases - metabolism
Down-Regulation - drug effects
Free Radical Scavengers - pharmacology
Gene Expression Regulation
Glutathione - metabolism
Humans
Melanins - biosynthesis
Melanoma, Experimental - metabolism
microphthalmia transcription factor
Microphthalmia-Associated Transcription Factor - genetics
Microphthalmia-Associated Transcription Factor - metabolism
Monophenol Monooxygenase - metabolism
Oxidation-Reduction
protein kinase A
reduced glutathione
tyrosinase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In our previous study, we showed that 4,4′-dihydroxybiphenyl (44′-BP) reduced melanin content via the inhibition of tyrosinase. In the current study, we utilized 44′-BP treated B16 melanoma cells (B16 cells) to measure several key cellular parameters known to be involved in melanogenic activity. Included in these measurements were tyrosinase and microphthalmia transcription factor (MITF) protein levels, cyclic AMP levels, protein kinase A (PKA) activation, and reduced glutathione (GSH) and oxidized glutathione (GSSG) levels. Results showed that 44′-BP effectively suppressed the amounts of tyrosinase and MITF proteins, cAMP levels, and PKA activation. In addition, 44′-BP enhanced the GSH/GSSG ratio. In conclusion, our data provide an evidence that 44′-BP suppressed several cellular key parameters in the melanogenic pathway by downregulating the cAMP-dependent PKA signaling pathway and decreasing MITF gene expression (implied from the reduced protein levels), which in turn suppressed tyrosinase. We propose that the antimelanogenic action of 44′-BP is likely carried out by a combined effect of its anti-oxidant property and its ability to enhance intracellular GSH levels.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.29.14