Mass production of somatic embryos expressing Escherichia coli heat-labile enterotoxin B subunit in Siberian ginseng

The B subunit of Escherichia coli heat-labile toxin (LTB) is a potent mucosal immunogen and immunoadjuvant for co-administered antigens. In order to produce large scale of LTB for the development of edible vaccine, we used transgenic somatic embryos of Siberian ginseng, which is known as medicinal p...

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Bibliographic Details
Published in:Journal of biotechnology 2006-01, Vol.121 (2), p.124-133
Main Authors: Kang, Tae-Jin, Lee, Won-Seok, Choi, Eun-Gyung, Kim, Jae-Whune, Kim, Bang-Geul, Yang, Moon-Sik
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - biosynthesis
Adjuvants, Immunologic - genetics
Agrobacterium tumefaciens
Agrobacterium-mediated transformation
Bacterial Toxins - biosynthesis
Bacterial Toxins - genetics
Bacterial Toxins - immunology
Bacterial Vaccines - biosynthesis
Bacterial Vaccines - genetics
Bacterial Vaccines - immunology
Biological and medical sciences
Bioreactor
bioreactors
Biotechnology
E. coli heat-labile enterotoxin
Edible vaccine
Eleutherococcus - embryology
Eleutherococcus - genetics
Eleutherococcus - immunology
Eleutherococcus senticosus
enterotoxins
Enterotoxins - biosynthesis
Enterotoxins - genetics
Enterotoxins - immunology
Escherichia coli
Escherichia coli - genetics
Escherichia coli - immunology
Escherichia coli Proteins - biosynthesis
Escherichia coli Proteins - genetics
Escherichia coli Proteins - immunology
Fundamental and applied biological sciences. Psychology
gene expression
gene transfer
genes
genetic transformation
Humans
Immunity, Mucosal - immunology
Plants, Genetically Modified - embryology
Plants, Genetically Modified - genetics
Plants, Genetically Modified - immunology
recombinant antigens
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Recombinant Proteins - immunology
Siberian ginseng
somatic embryogenesis
somatic embryos
transgenic plants
vaccine development
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Summary:The B subunit of Escherichia coli heat-labile toxin (LTB) is a potent mucosal immunogen and immunoadjuvant for co-administered antigens. In order to produce large scale of LTB for the development of edible vaccine, we used transgenic somatic embryos of Siberian ginseng, which is known as medicinal plant. When transgenic somatic embryos were cultured in 130 L air-lift type bioreactor, they were developed to mature somatic embryos through somatic embryogenesis and contained approximately 0.36% LTB of the total soluble protein. Enzyme-linked immunosorbent assay indicated that the somatic embryo-synthesized LTB protein bound specifically to G M1-ganglioside, suggesting the LTB subunits formed active pentamers. Therefore, the use of the bioreactor system for expression of LTB proteins in somatic embryos allows for continuous mass production in a short-term period.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2005.07.020